Detailed information of TA system TA224643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 104217..104731
• Replicon: plasmid P1
• Accession: NZ_CP087003
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-105

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE06_RS19745
• Protein ID: WP_224556955.1
• Coordinates: 104465..104731 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOE06_RS19740
• Protein ID: WP_224556953.1
• Coordinates: 104217..104468 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE06_RS19720 (LOE06_19720)	100474..100830	-	357	WP_224556951.1	hypothetical protein	-
LOE06_RS19725 (LOE06_19725)	101072..101563	-	492	WP_224557005.1	3-keto-5-aminohexanoate cleavage protein	-
LOE06_RS19730 (LOE06_19730)	101729..102673	+	945	Protein_102	transposase	-
LOE06_RS19735 (LOE06_19735)	102674..103585	+	912	Protein_103	IS3 family transposase	-
LOE06_RS19740 (LOE06_19740)	104217..104468	+	252	WP_224556953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE06_RS19745 (LOE06_19745)	104465..104731	+	267	WP_224556955.1	Txe/YoeB family addiction module toxin	Toxin
LOE06_RS19750	104792..105610	-	819	WP_229940646.1	hypothetical protein	-
LOE06_RS19755 (LOE06_19755)	105701..106404	+	704	Protein_107	IS6 family transposase	-
LOE06_RS19760 (LOE06_19760)	106622..107479	-	858	WP_229940775.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / fcl	1..281161	281161
	-	flank	IS/Tn	-	-	102758..103585	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.78 Da
• Isoelectric Point: 9.5014

>T224643 WP_224556955.1 NZ_CP087003:104465-104731 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVNTNDKVRDRINELIKQCRRTPFKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224643 NZ_CP120376:c5861181-5860417 [Pseudomonas nitroreducens]
ATGTCCTTCGAAGCCGGCGCCCGTCTATGGGCGAAGTATGAGGACTCGGTTGTCGCGCCCATCCGTGGGCGCCTGGTGCG
ACTGGTGGAGAGCCAGGGCCAGGTGGCGACGTTGCAACTGGTGGACACGCTGGAAGAGCAGGCACTGCTGGAGGAGTTGC
TGGAAACCAGCAAACCGCGCATGCCGTCCAGCGCCGAGCCACTGCATTACCTGCTGAAGACACCGTTCCGCTACCCGCCG
CTGCGCTGGGGCTCGCGCTTTGGTCGGCGCCATGAGCCGAGCCTGTTCTACGGCGCGCTCAAGCTGGAAACGGCGATGGC
GGAATCGGCGTTCTACCGCTTCGTGCTGTGGGAGGGGATGGCCACGCCGCCGCCCAGTGGACGGATCCTTTCCGAGCACG
CTTCCTTCGAGGCGCGCTTCCAGGTGCAGAAGGGCGTGCGGCTGCACCTGCCGCCGTTCCGCGAGCACGCGGCCCTGCTT
GATCCACAGGATTACAGCGTGACTCAGGGGATGGGCAGCGCGATGCGCGATGCAGGGGTGGAGGCTTTCGAATATCGTTC
GGCGCGTTGTCCGCAGGGGGGCATAAATGTGGCGTTGTTCGTGCCCTCGGCCTTTACCGAGAAGCGCCCGCGCAACCTGA
TGCCCTGGCTTTGCGAGACGACTTCGGAGTACGTCGCCTTCAAGCATGCCCAGGTGCCCGACAGCCCCCGCATTTACCGG
CGTGAACAGTACCTGGTGGATGGCAGGCTACCTCATCCTGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.43 Da
• Isoelectric Point: 6.4652

>AT224643 WP_224556953.1 NZ_CP087003:104217-104468 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLETWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEVRE
LTE

Nucleotide

• Length: 252 bp

>AT224643 NZ_CP120376:c5861550-5861182 [Pseudomonas nitroreducens]
ATGAGCGCACTGCTGAAGGAAAACCCGGCTGCCGATACCGTCCTCGCGAAGGCGGTGTTGACTGCCCGCGAGCACCTGGC
GATGACACAGCAGGAGCTGGCTGCCATTGTCGGCGTGGACCGCAGCGCGGTGAGCCGCTGGAAGCAGAATGGCCTGCGCG
TGGACAGCAAGACCGGCGAGCTGGCGTTGCTGCTGGTGCGCATCTACCGCGCGCTGTTCGCTCTGTTCGGCGGCAATCAT
GAGGACATGCGCCACTTCCTGCGCACCGAGAACCGCCACCTGGGTGGTGTGCCGCTGCAGTTGATGGAGCGGGTGCAGGG
GCTGGTCGCAGTGGTCGAGTACCTGGACGCCATCCGGGGCAAGGTCTGA