Detailed information of TA system TA224618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 92528..93042
• Replicon: plasmid P2
• Accession: NZ_CP086973
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-114

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE10_RS20045
• Protein ID: WP_224556955.1
• Coordinates: 92776..93042 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOE10_RS20040
• Protein ID: WP_224556953.1
• Coordinates: 92528..92779 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE10_RS20020 (LOE10_20025)	88785..89141	-	357	WP_224556951.1	hypothetical protein	-
LOE10_RS20025 (LOE10_20030)	89383..89874	-	492	WP_224557005.1	3-keto-5-aminohexanoate cleavage protein	-
LOE10_RS20030 (LOE10_20035)	90040..90984	+	945	Protein_88	transposase	-
LOE10_RS20035 (LOE10_20040)	90985..91896	+	912	Protein_89	IS3 family transposase	-
LOE10_RS20040 (LOE10_20045)	92528..92779	+	252	WP_224556953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE10_RS20045 (LOE10_20050)	92776..93042	+	267	WP_224556955.1	Txe/YoeB family addiction module toxin	Toxin
LOE10_RS20050 (LOE10_20055)	93103..93921	-	819	WP_229940646.1	hypothetical protein	-
LOE10_RS20055 (LOE10_20060)	94012..94715	+	704	Protein_93	IS6 family transposase	-
LOE10_RS20060 (LOE10_20065)	94933..95790	-	858	WP_229940775.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / fcl	1..281177	281177
	-	flank	IS/Tn	-	-	91069..91896	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.78 Da
• Isoelectric Point: 9.5014

>T224618 WP_224556955.1 NZ_CP086973:92776-93042 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVNTNDKVRDRINELIKQCRRTPFKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224618 NZ_CP120366:c3815-3396 [Sinorhizobium kummerowiae]
GTGAGCAGGCTCTACATGCTGGACACCGATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTGCTGGCGCAAATCGAGACCATTCTTGAAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGGAGGCCCATCGGCCCGAATGACCTGTTCATCGCGGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCATTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTTCTTCGGGTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.43 Da
• Isoelectric Point: 6.4652

>AT224618 WP_224556953.1 NZ_CP086973:92528-92779 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLETWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEVRE
LTE

Nucleotide

• Length: 252 bp

>AT224618 NZ_CP120366:c4081-3812 [Sinorhizobium kummerowiae]
ATGCCCCAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCCAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAAGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCTGACGTTGACGAAACC
CTGTTGCCGGTGAAGGAAATCGACCTGTGA