Detailed information of TA system TA224615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 23088..23602
• Replicon: plasmid P7
• Accession: NZ_CP086970
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-15

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE11_RS22935
• Protein ID: WP_224557656.1
• Coordinates: 23088..23354 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE11_RS22940
• Protein ID: WP_036743750.1
• Coordinates: 23351..23602 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE11_RS22915 (LOE11_22915)	19117..20151	+	1035	WP_151654626.1	replication initiator protein A	-
LOE11_RS22920 (LOE11_22920)	20266..20934	+	669	WP_151654624.1	ParA family protein	-
LOE11_RS22925 (LOE11_22925)	21577..21768	+	192	WP_224557658.1	hypothetical protein	-
LOE11_RS22930 (LOE11_22930)	21802..22845	+	1044	WP_224557657.1	IS5 family transposase	-
LOE11_RS22935 (LOE11_22935)	23088..23354	-	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE11_RS22940 (LOE11_22940)	23351..23602	-	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE11_RS22945 (LOE11_22945)	23709..24395	-	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE11_RS22950 (LOE11_22950)	24567..25631	-	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE11_RS22955 (LOE11_22955)	25969..27150	+	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE11_RS22960 (LOE11_22960)	27169..28080	+	912	WP_229940889.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37720	37720
	-	flank	IS/Tn	-	-	21802..22845	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224615 WP_224557656.1 NZ_CP086970:c23354-23088 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224615 NZ_CP120365:c3155151-3154765 [Sinorhizobium kummerowiae]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCAGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTGTTCAAGGGAAGTGATTTCTCGCAAACCGATATCCCGGCGGCGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224615 WP_036743750.1 NZ_CP086970:c23602-23351 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224615 NZ_CP120365:c3155405-3155151 [Sinorhizobium kummerowiae]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGCACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AAGCCATTCAACGCCGCTGGAATGCCATGCCCGTCCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5