Detailed information of TA system TA224592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 86158..86672
• Replicon: plasmid P3
• Accession: NZ_CP086941
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-29

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE15_RS20045
• Protein ID: WP_224556955.1
• Coordinates: 86406..86672 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOE15_RS20040
• Protein ID: WP_224556953.1
• Coordinates: 86158..86409 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE15_RS20020 (LOE15_19980)	82415..82771	-	357	WP_224556951.1	hypothetical protein	-
LOE15_RS20025 (LOE15_19985)	83013..83438	-	426	WP_229939140.1	3-keto-5-aminohexanoate cleavage protein	-
LOE15_RS20030 (LOE15_19990)	83670..84614	+	945	Protein_82	transposase	-
LOE15_RS20035 (LOE15_19995)	84615..85526	+	912	Protein_83	IS3 family transposase	-
LOE15_RS20040 (LOE15_20000)	86158..86409	+	252	WP_224556953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE15_RS20045 (LOE15_20005)	86406..86672	+	267	WP_224556955.1	Txe/YoeB family addiction module toxin	Toxin
LOE15_RS20050	86733..87551	-	819	WP_229940646.1	hypothetical protein	-
LOE15_RS20055 (LOE15_20015)	87642..88345	+	704	Protein_87	IS6 family transposase	-
LOE15_RS20060 (LOE15_20020)	88563..89444	-	882	WP_224556958.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..168033	168033
	-	flank	IS/Tn	-	-	84699..85526	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.78 Da
• Isoelectric Point: 9.5014

>T224592 WP_224556955.1 NZ_CP086941:86406-86672 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVNTNDKVRDRINELIKQCRRTPFKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224592 NZ_CP120359:6579-6866 [Pseudomonas sp. AA-38]
ATGACCTATAGCCTCGAATTCGATGCACGAGCACTTAAGGAGTGGCACAAGCTGGGGGACACCGTCCGCGAGCAGTTCAA
GAAAAAGCTTGCGGAGGTTCTGGTCAACCCGCGCGTCGAGGCGAATCGCCTTCACTCGCTACCCGACTGCTACAAGATCA
AGCTGCGCAGCAGTGGTTATCGCCTGGTCTATCAGGTGATCGACCATGAGGTGGTGGTGTTTGTCGTCGCGGTGGATCGA
CGTGACCGTGAGCAGGCTTACCTCAAGGCCGCTGAGCGGCTCAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.43 Da
• Isoelectric Point: 6.4652

>AT224592 WP_224556953.1 NZ_CP086941:86158-86409 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLETWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEVRE
LTE

Nucleotide

• Length: 252 bp

>AT224592 NZ_CP120359:6350-6589 [Pseudomonas sp. AA-38]
ATGGCCTCTATCAACATCCGTATCGACGATGAACTGAAAGAGCGGGCTTACCGTGAACTGGAAAAGCTCGGTGTCACGCC
TTCCGAGCTGATGCGCCAAACCTTGCAGTATGTTGCCGAGCGCGGCCAGTTGCCTTTCCGTCCGGTGTTGTTGACCGAGG
AGGATGAAGCCTTGATCGCCACCGTTCGTGAGCGCCTCGAAACGCCTCAGCGAGTGAAGGTTTCGCTGGATGACCTATAG