Detailed information of TA system TA224591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 10723..11237
• Replicon: plasmid P1
• Accession: NZ_CP086939
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-29

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE15_RS19315
• Protein ID: WP_224557656.1
• Coordinates: 10971..11237 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE15_RS19310
• Protein ID: WP_036743750.1
• Coordinates: 10723..10974 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE15_RS19290 (LOE15_19260)	6245..7156	-	912	WP_229940889.1	branched-chain amino acid ABC transporter permease	-
LOE15_RS19295 (LOE15_19265)	7175..8356	-	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE15_RS19300 (LOE15_19270)	8694..9758	+	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE15_RS19305 (LOE15_19275)	9930..10616	+	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE15_RS19310 (LOE15_19280)	10723..10974	+	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE15_RS19315 (LOE15_19285)	10971..11237	+	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE15_RS19320 (LOE15_19290)	11480..12523	-	1044	WP_224557657.1	IS5 family transposase	-
LOE15_RS19325 (LOE15_19295)	12557..12748	-	192	WP_224557658.1	hypothetical protein	-
LOE15_RS19330 (LOE15_19300)	13391..14044	-	654	WP_229941983.1	ParA family protein	-
LOE15_RS19335 (LOE15_19305)	14174..15208	-	1035	WP_151654626.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37724	37724
	-	flank	IS/Tn	-	-	11480..12523	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224591 WP_224557656.1 NZ_CP086939:10971-11237 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224591 NZ_CP120356:c2769412-2768843 [Rhodococcus rhodochrous]
ATGAGGCGTCTGGATCTGATCGTCGGTCCCAATGGTTCCGGCAAGTCGACGTTCGTCGAGTTGTCCCTCGCTCCCCTGCT
GCCGCGCAGTGTGTTCGTCAACGCGGATGTGATCGCGCGGCAACGCTGGCCGGACCGTGCCGAGGAAATGTCCTACGAGG
CGGCGCAACTCGCCGCGGCGGCGCGGGATCGATTGCTGCGGCAGGGCCGGTCGTTCATCGCGGAGACGGTGTTCTCGCAT
CCGTCGAAGCTGGAGCTGATCGACCGCGCACACGAGGCGGGCTTCACGGTGGTGTTGCACGTGATGCTGGTGCCCGAGGC
GATGTCGGTGCTGCGTGTGCAGCACCGGGTGGCCTCCGGTGGGCATGCGGTGCCCGAGGACAAGATCCGGGCGCGTTATC
GGCGGTTGTGGCCGCTGGTGGCCGAGGCGATCGGCCGCGCCGATCAGGCGTCGGTGTACGACACGGCGTCCGGGACCCCG
GTGGTGGTGGCGCGGTTCGCCGGCGGGGTGCCGGTGGGTGCGGTGCGCTGGCCGGGGTGGACGCCGGCGGCGCTCACCGG
GCTCGGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224591 WP_036743750.1 NZ_CP086939:10723-10974 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224591 NZ_CP120356:c2769795-2769409 [Rhodococcus rhodochrous]
ATGACGTCGTCCACCGACAAGGTCACCCGCTTCGACGCCGAACTCGTCGACAGCGCCATCGCGGAGGGCGGCCGCCAGAA
TCGCACCGGACGCCAGCAACTCGAATACTGGGCCCGTATCGGCCGCGCGATGACCGCCCATGAGACCGCCTCCCTGCACC
GGGTGCACGAGGCGCTGGCCGGGACGCGGGAGCTGAGCGAACTCACCGCTGCCGAGGGACGGCTGTTCGACGCGGAGATC
GATGCGCGCCTGGCGGACGGGCTGGCCCACACCGATTACGCCGAGGTGCTCGCCGCGCGTGGGGTCACCACGGTGGTGCT
CGACGACGAGGGCCGGTTGGTCGAGCAGGGTCCCGACGGTAGTCGCCGGGTGCTCGACGACGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5