22458

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2271874..2272376 Replicon chromosome
Accession NC_009801
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECE24377A_RS13170 Protein ID WP_000767829.1
Coordinates 2271874..2272128 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECE24377A_RS13175 Protein ID WP_001259255.1
Coordinates 2272125..2272376 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS13145 2266889..2267116 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECE24377A_RS13150 2267130..2268188 - 1059 WP_000492341.1 transport protein YeeE -
ECE24377A_RS13155 2268367..2269725 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECE24377A_RS30035 2269715..2269777 - 63 WP_010723108.1 membrane protein YoeI -
ECE24377A_RS13160 2269992..2270921 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECE24377A_RS13165 2270967..2271791 - 825 WP_000754758.1 SDR family oxidoreductase -
ECE24377A_RS13170 2271874..2272128 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECE24377A_RS13175 2272125..2272376 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECE24377A_RS27965 2272659..2272709 + 51 WP_001364200.1 his operon leader peptide -
ECE24377A_RS13180 2272855..2273754 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECE24377A_RS13185 2273760..2275064 + 1305 WP_012138710.1 histidinol dehydrogenase -
ECE24377A_RS13190 2275061..2276131 + 1071 WP_000108956.1 histidinol-phosphate transaminase -
ECE24377A_RS13195 2276131..2277198 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T22458 WP_000767829.1 NC_009801:c2272128-2271874 [Escherichia coli O139:H28 str. E24377A]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T22458 NC_009801:c2272128-2271874 [Escherichia coli O139:H28 str. E24377A]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT22458 WP_001259255.1 NC_009801:c2272376-2272125 [Escherichia coli O139:H28 str. E24377A]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT22458 NC_009801:c2272376-2272125 [Escherichia coli O139:H28 str. E24377A]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References