22458

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2271874..2272376 Replicon chromosome
Accession NC_009801
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECE24377A_RS13170 Protein ID WP_000767829.1
Coordinates 2271874..2272128 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECE24377A_RS13175 Protein ID WP_001259255.1
Coordinates 2272125..2272376 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS13145 2266889..2267116 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECE24377A_RS13150 2267130..2268188 - 1059 WP_000492341.1 transport protein YeeE -
ECE24377A_RS13155 2268367..2269725 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECE24377A_RS30035 2269715..2269777 - 63 WP_010723108.1 membrane protein YoeI -
ECE24377A_RS13160 2269992..2270921 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECE24377A_RS13165 2270967..2271791 - 825 WP_000754758.1 SDR family oxidoreductase -
ECE24377A_RS13170 2271874..2272128 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECE24377A_RS13175 2272125..2272376 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECE24377A_RS27965 2272659..2272709 + 51 WP_001364200.1 his operon leader peptide -
ECE24377A_RS13180 2272855..2273754 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECE24377A_RS13185 2273760..2275064 + 1305 WP_012138710.1 histidinol dehydrogenase -
ECE24377A_RS13190 2275061..2276131 + 1071 WP_000108956.1 histidinol-phosphate transaminase -
ECE24377A_RS13195 2276131..2277198 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T22458 WP_000767829.1 NC_009801:c2272128-2271874 [Escherichia coli O139:H28 str. E24377A]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T22458 NC_009801:c2272128-2271874 [Escherichia coli O139:H28 str. E24377A]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT22458 WP_001259255.1 NC_009801:c2272376-2272125 [Escherichia coli O139:H28 str. E24377A]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT22458 NC_009801:c2272376-2272125 [Escherichia coli O139:H28 str. E24377A]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References