Detailed information of TA system TA224578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 188136..188650
• Replicon: plasmid P3
• Accession: NZ_CP086922
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-32

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE17_RS20665
• Protein ID: WP_224556955.1
• Coordinates: 188136..188402 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOE17_RS20670
• Protein ID: WP_224556953.1
• Coordinates: 188399..188650 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE17_RS20650 (LOE17_20625)	185364..186245	+	882	WP_224556958.1	zinc-binding dehydrogenase	-
LOE17_RS20655 (LOE17_20630)	186463..187166	-	704	Protein_182	IS6 family transposase	-
LOE17_RS20660 (LOE17_20635)	187257..188075	+	819	WP_229940646.1	hypothetical protein	-
LOE17_RS20665 (LOE17_20640)	188136..188402	-	267	WP_224556955.1	Txe/YoeB family addiction module toxin	Toxin
LOE17_RS20670 (LOE17_20645)	188399..188650	-	252	WP_224556953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE17_RS20675 (LOE17_20650)	189282..190193	-	912	Protein_186	IS3 family transposase	-
LOE17_RS20680 (LOE17_20655)	190194..191138	-	945	Protein_187	transposase	-
LOE17_RS20685 (LOE17_20660)	191304..191795	+	492	WP_224557005.1	3-keto-5-aminohexanoate cleavage protein	-
LOE17_RS20690 (LOE17_20665)	192037..192393	+	357	WP_224556951.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fcl / gmd	1..281177	281177
	-	flank	IS/Tn	-	-	189282..190109	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.78 Da
• Isoelectric Point: 9.5014

>T224578 WP_224556955.1 NZ_CP086922:c188402-188136 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVNTNDKVRDRINELIKQCRRTPFKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224578 NZ_CP120349:2101796-2101899 [Salmonella enterica subsp. enterica serovar Muenchen]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.43 Da
• Isoelectric Point: 6.4652

>AT224578 WP_224556953.1 NZ_CP086922:c188650-188399 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLETWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEVRE
LTE

Nucleotide

• Length: 252 bp

>AT224578 NZ_CP120349:c2101901-2101756 [Salmonella enterica subsp. enterica serovar Muenchen]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG