Detailed information of TA system TA224577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 22176..22690
• Replicon: plasmid P1
• Accession: NZ_CP086920
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-32

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE17_RS19365
• Protein ID: WP_224557656.1
• Coordinates: 22176..22442 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE17_RS19370
• Protein ID: WP_036743750.1
• Coordinates: 22439..22690 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE17_RS19345 (LOE17_19325)	18205..19239	+	1035	WP_151654626.1	replication initiator protein A	-
LOE17_RS19350 (LOE17_19330)	19369..20022	+	654	WP_229941983.1	ParA family protein	-
LOE17_RS19355 (LOE17_19335)	20665..20856	+	192	WP_224557658.1	hypothetical protein	-
LOE17_RS19360 (LOE17_19340)	20890..21933	+	1044	WP_224557657.1	IS5 family transposase	-
LOE17_RS19365 (LOE17_19345)	22176..22442	-	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE17_RS19370 (LOE17_19350)	22439..22690	-	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE17_RS19375 (LOE17_19355)	22797..23483	-	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE17_RS19380 (LOE17_19360)	23655..24719	-	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE17_RS19385 (LOE17_19365)	25057..26238	+	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE17_RS19390 (LOE17_19370)	26257..27168	+	912	WP_229940889.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37726	37726
	-	flank	IS/Tn	-	-	20890..21933	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224577 WP_224557656.1 NZ_CP086920:c22442-22176 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224577 NZ_CP120349:c1017848-1017321 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224577 WP_036743750.1 NZ_CP086920:c22690-22439 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224577 NZ_CP120349:c1018135-1017845 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5