Detailed information of TA system TA224574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 25280..25794
• Replicon: plasmid P9
• Accession: NZ_CP086918
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-34

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE18_RS24750
• Protein ID: WP_224557656.1
• Coordinates: 25280..25546 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE18_RS24755
• Protein ID: WP_036743750.1
• Coordinates: 25543..25794 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE18_RS24730 (LOE18_24660)	21309..22343	+	1035	WP_151654626.1	replication initiator protein A	-
LOE18_RS24735 (LOE18_24665)	22473..23126	+	654	WP_229941983.1	ParA family protein	-
LOE18_RS24740 (LOE18_24670)	23769..23960	+	192	WP_224557658.1	hypothetical protein	-
LOE18_RS24745 (LOE18_24675)	23994..25037	+	1044	WP_224557657.1	IS5 family transposase	-
LOE18_RS24750 (LOE18_24680)	25280..25546	-	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE18_RS24755 (LOE18_24685)	25543..25794	-	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE18_RS24760 (LOE18_24690)	25901..26587	-	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE18_RS24765 (LOE18_24695)	26759..27823	-	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE18_RS24770 (LOE18_24700)	28161..29342	+	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE18_RS24775 (LOE18_24705)	29361..30272	+	912	WP_229940889.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37758	37758
	-	flank	IS/Tn	-	-	23994..25037	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224574 WP_224557656.1 NZ_CP086918:c25546-25280 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224574 NZ_CP120348:50116-50421 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224574 WP_036743750.1 NZ_CP086918:c25794-25543 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224574 NZ_CP120348:49896-50114 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5