Detailed information of TA system TA224567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 17924..18438
• Replicon: plasmid P7
• Accession: NZ_CP086908
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-35

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE19_RS24245
• Protein ID: WP_224557656.1
• Coordinates: 17924..18190 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE19_RS24250
• Protein ID: WP_036743750.1
• Coordinates: 18187..18438 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE19_RS24225 (LOE19_24225)	13953..14987	+	1035	WP_151654626.1	replication initiator protein A	-
LOE19_RS24230 (LOE19_24230)	15102..15770	+	669	WP_151654624.1	ParA family protein	-
LOE19_RS24235 (LOE19_24235)	16413..16604	+	192	WP_224557658.1	hypothetical protein	-
LOE19_RS24240 (LOE19_24240)	16638..17681	+	1044	WP_224557657.1	IS5 family transposase	-
LOE19_RS24245 (LOE19_24245)	17924..18190	-	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE19_RS24250 (LOE19_24250)	18187..18438	-	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE19_RS24255 (LOE19_24255)	18545..19231	-	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE19_RS24260 (LOE19_24260)	19403..20467	-	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE19_RS24265 (LOE19_24265)	20805..21986	+	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE19_RS24270 (LOE19_24270)	22059..22916	+	858	WP_224557653.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32556	32556
	-	flank	IS/Tn	-	-	16638..17681	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224567 WP_224557656.1 NZ_CP086908:c18190-17924 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224567 NZ_CP120347:c4165975-4165637 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAATCCTTTCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTACTTGTCTGGCAAACGCTACTGACACG
CCTGCTGGAGCAACACTATGGCCTGTCGCTGAACGATACGCCGTTCAGTGATGAAACGGTTATTCAGGAGCATATCAACG
CCGGTATCACGCTGGCGGATGCAGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAACAGTCGCCTTACCTTCGGGCGGTTGATATTTTACGGGCGCGACAGGCTACTGGTCTGTTGCGGCAAAACCA
GCTTCCTTCGACACGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224567 WP_036743750.1 NZ_CP086908:c18438-18187 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224567 NZ_CP120347:c4166329-4166000 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGAAACCGACACAGTACAGTGGGGACTCAACCGACCTGGTTCTCCTCAATTTAGCGCACGCCTGGTACAAGA
AGGCAAACGCCTACATTATTTAGCTGACCGCGCCGGGATAACAGGCGCTTTCAATGAAGAAGAGTGTCACCAACTGAATG
CCGCATTTCCGCACTTCGTAAAACAAATGGAGCTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCATATCGCGTA
ACGCTGTACCACAACGAACTGACCTGTGAAGCAGATACGCTCGGTAGCTGTGGCTACGTTTATATAGCGATTTATCCCAC
TCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5