Detailed information of TA system TA224564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 103768..104282
• Replicon: plasmid P2
• Accession: NZ_CP086903
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-35

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE19_RS21200
• Protein ID: WP_224556955.1
• Coordinates: 104016..104282 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOE19_RS21195
• Protein ID: WP_224556953.1
• Coordinates: 103768..104019 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE19_RS21175 (LOE19_21170)	100025..100381	-	357	WP_224556951.1	hypothetical protein	-
LOE19_RS21180 (LOE19_21175)	100623..101114	-	492	WP_224557005.1	3-keto-5-aminohexanoate cleavage protein	-
LOE19_RS21185 (LOE19_21180)	101280..102224	+	945	Protein_101	transposase	-
LOE19_RS21190 (LOE19_21185)	102225..103136	+	912	Protein_102	IS3 family transposase	-
LOE19_RS21195 (LOE19_21190)	103768..104019	+	252	WP_224556953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE19_RS21200 (LOE19_21195)	104016..104282	+	267	WP_224556955.1	Txe/YoeB family addiction module toxin	Toxin
LOE19_RS21205	104343..105161	-	819	WP_229940646.1	hypothetical protein	-
LOE19_RS21210 (LOE19_21205)	105252..105955	+	704	Protein_106	IS6 family transposase	-
LOE19_RS21215 (LOE19_21210)	106173..107054	-	882	WP_224556958.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..149480	149480
	-	flank	IS/Tn	-	-	102309..103136	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.78 Da
• Isoelectric Point: 9.5014

>T224564 WP_224556955.1 NZ_CP086903:104016-104282 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVNTNDKVRDRINELIKQCRRTPFKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224564 NZ_CP120347:2101795-2101898 [Salmonella enterica subsp. enterica serovar Muenchen]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.43 Da
• Isoelectric Point: 6.4652

>AT224564 WP_224556953.1 NZ_CP086903:103768-104019 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLETWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEVRE
LTE

Nucleotide

• Length: 252 bp

>AT224564 NZ_CP120347:c2101900-2101755 [Salmonella enterica subsp. enterica serovar Muenchen]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG