Detailed information of TA system TA224543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 32711..33225
• Replicon: plasmid P1
• Accession: NZ_CP086878
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-89

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE22_RS19410
• Protein ID: WP_224557656.1
• Coordinates: 32959..33225 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE22_RS19405
• Protein ID: WP_036743750.1
• Coordinates: 32711..32962 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE22_RS19385 (LOE22_19390)	28233..29144	-	912	WP_229940889.1	branched-chain amino acid ABC transporter permease	-
LOE22_RS19390 (LOE22_19395)	29163..30344	-	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE22_RS19395 (LOE22_19400)	30682..31746	+	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE22_RS19400 (LOE22_19405)	31918..32604	+	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE22_RS19405 (LOE22_19410)	32711..32962	+	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE22_RS19410 (LOE22_19415)	32959..33225	+	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE22_RS19415 (LOE22_19420)	33468..34511	-	1044	WP_224557657.1	IS5 family transposase	-
LOE22_RS19420 (LOE22_19425)	34545..34736	-	192	WP_224557658.1	hypothetical protein	-
LOE22_RS19425 (LOE22_19430)	35379..36047	-	669	WP_151654624.1	ParA family protein	-
LOE22_RS19430 (LOE22_19435)	36162..37196	-	1035	WP_151654626.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37728	37728
	-	flank	IS/Tn	-	-	33468..34511	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224543 WP_224557656.1 NZ_CP086878:32959-33225 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224543 NZ_CP120344:14788-15120 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224543 WP_036743750.1 NZ_CP086878:32711-32962 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224543 NZ_CP120344:14530-14787 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5