Detailed information of TA system TA224538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 92527..93041
• Replicon: plasmid P3
• Accession: NZ_CP086873
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-97

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE23_RS20510
• Protein ID: WP_224556955.1
• Coordinates: 92775..93041 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOE23_RS20505
• Protein ID: WP_224556953.1
• Coordinates: 92527..92778 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE23_RS20485 (LOE23_20470)	88784..89140	-	357	WP_224556951.1	hypothetical protein	-
LOE23_RS20490 (LOE23_20475)	89382..89873	-	492	WP_224557005.1	3-keto-5-aminohexanoate cleavage protein	-
LOE23_RS20495 (LOE23_20480)	90039..90983	+	945	Protein_88	transposase	-
LOE23_RS20500 (LOE23_20485)	90984..91895	+	912	Protein_89	IS3 family transposase	-
LOE23_RS20505 (LOE23_20490)	92527..92778	+	252	WP_224556953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE23_RS20510 (LOE23_20495)	92775..93041	+	267	WP_224556955.1	Txe/YoeB family addiction module toxin	Toxin
LOE23_RS20515 (LOE23_20500)	93102..93920	-	819	WP_229940646.1	hypothetical protein	-
LOE23_RS20520 (LOE23_20505)	94011..94714	+	704	Protein_93	IS6 family transposase	-
LOE23_RS20525 (LOE23_20510)	94932..95789	-	858	WP_229940775.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / fcl	1..281181	281181
	-	flank	IS/Tn	-	-	91068..91895	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.78 Da
• Isoelectric Point: 9.5014

>T224538 WP_224556955.1 NZ_CP086873:92775-93041 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVNTNDKVRDRINELIKQCRRTPFKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224538 NZ_CP120343:3723631-3723906 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGAATGCCTCCGGCGTTAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.43 Da
• Isoelectric Point: 6.4652

>AT224538 WP_224556953.1 NZ_CP086873:92527-92778 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLETWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEVRE
LTE

Nucleotide

• Length: 252 bp

>AT224538 NZ_CP120343:c3723896-3723474 [Salmonella enterica subsp. enterica serovar Paratyphi B]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTAACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT