Detailed information of TA system TA224512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 103766..104280
• Replicon: plasmid P2
• Accession: NZ_CP086841
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-31

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE24_RS21220
• Protein ID: WP_224556955.1
• Coordinates: 104014..104280 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LOE24_RS21215
• Protein ID: WP_224556953.1
• Coordinates: 103766..104017 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE24_RS21195 (LOE24_21165)	100023..100379	-	357	WP_224556951.1	hypothetical protein	-
LOE24_RS21200 (LOE24_21170)	100621..101112	-	492	WP_224557005.1	3-keto-5-aminohexanoate cleavage protein	-
LOE24_RS21205 (LOE24_21175)	101278..102222	+	945	Protein_101	transposase	-
LOE24_RS21210 (LOE24_21180)	102223..103134	+	912	Protein_102	IS3 family transposase	-
LOE24_RS21215 (LOE24_21185)	103766..104017	+	252	WP_224556953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE24_RS21220 (LOE24_21190)	104014..104280	+	267	WP_224556955.1	Txe/YoeB family addiction module toxin	Toxin
LOE24_RS21225 (LOE24_21195)	104341..105159	-	819	WP_229940646.1	hypothetical protein	-
LOE24_RS21230 (LOE24_21200)	105250..105953	+	704	Protein_106	IS6 family transposase	-
LOE24_RS21235 (LOE24_21205)	106171..107052	-	882	WP_224556958.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / fcl	1..281165	281165
	-	flank	IS/Tn	-	-	102307..103134	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.78 Da
• Isoelectric Point: 9.5014

>T224512 WP_224556955.1 NZ_CP086841:104014-104280 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVNTNDKVRDRINELIKQCRRTPFKGTGKPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224512 NZ_CP120339:2101800-2101903 [Salmonella enterica subsp. enterica serovar Muenchen]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.43 Da
• Isoelectric Point: 6.4652

>AT224512 WP_224556953.1 NZ_CP086841:103766-104017 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLETWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEVRE
LTE

Nucleotide

• Length: 252 bp

>AT224512 NZ_CP120339:c2101905-2101760 [Salmonella enterica subsp. enterica serovar Muenchen]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG