Detailed information of TA system TA224489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 22057..22571
• Replicon: plasmid P7
• Accession: NZ_CP086815
• Organism: Pseudosulfitobacter pseudonitzschiae strain TC12-54

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE27_RS24040
• Protein ID: WP_224557656.1
• Coordinates: 22057..22323 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE27_RS24045
• Protein ID: WP_036743750.1
• Coordinates: 22320..22571 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE27_RS24020 (LOE27_24000)	18086..19120	+	1035	WP_151654626.1	replication initiator protein A	-
LOE27_RS24025 (LOE27_24005)	19235..19903	+	669	WP_151654624.1	ParA family protein	-
LOE27_RS24030 (LOE27_24010)	20546..20737	+	192	WP_224557658.1	hypothetical protein	-
LOE27_RS24035 (LOE27_24015)	20771..21814	+	1044	WP_224557657.1	IS5 family transposase	-
LOE27_RS24040 (LOE27_24020)	22057..22323	-	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE27_RS24045 (LOE27_24025)	22320..22571	-	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE27_RS24050 (LOE27_24030)	22678..23364	-	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE27_RS24055 (LOE27_24035)	23536..24600	-	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE27_RS24060 (LOE27_24040)	24938..26119	+	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE27_RS24065 (LOE27_24045)	26138..27049	+	912	WP_229940889.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37721	37721
	-	flank	IS/Tn	-	-	20771..21814	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224489 WP_224557656.1 NZ_CP086815:c22323-22057 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224489 NZ_CP120335:c4666351-4666040 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224489 WP_036743750.1 NZ_CP086815:c22571-22320 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224489 NZ_CP120335:c4666039-4665749 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5