Detailed information of TA system TA224448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 60688..61415
• Replicon: plasmid pAZS099-NDM-IMP
• Accession: NZ_CP086762
• Organism: Klebsiella pneumoniae strain AZS099

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LON84_RS25560
• Protein ID: WP_011251285.1
• Coordinates: 61104..61415 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LON84_RS25555
• Protein ID: WP_011251286.1
• Coordinates: 60688..61107 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LON84_RS25530 (LON84_25535)	56281..57648	+	1368	WP_017900870.1	formimidoylglutamate deiminase	-
LON84_RS25535 (LON84_25540)	58173..58708	+	536	Protein_63	transposase	-
LON84_RS25540 (LON84_25545)	58822..59088	-	267	WP_017900868.1	hypothetical protein	-
LON84_RS25545 (LON84_25550)	59192..60159	+	968	Protein_65	IS5-like element IS903B family transposase	-
LON84_RS25550 (LON84_25555)	60205..60618	-	414	WP_017901337.1	hypothetical protein	-
LON84_RS25555 (LON84_25560)	60688..61107	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LON84_RS25560 (LON84_25565)	61104..61415	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LON84_RS25565 (LON84_25570)	61620..62057	-	438	Protein_69	DDE-type integrase/transposase/recombinase	-
LON84_RS25570 (LON84_25575)	62171..62548	+	378	WP_004118218.1	transposase	-
LON84_RS25575 (LON84_25580)	62545..62892	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
LON84_RS25580 (LON84_25585)	62941..64479	+	1539	WP_017901237.1	IS66 family transposase	-
LON84_RS25585 (LON84_25590)	64519..64698	+	180	Protein_73	IS5/IS1182 family transposase	-
LON84_RS25590 (LON84_25595)	65244..66224	-	981	WP_000019441.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaIMP-4 / blaSHV-12 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / sul2	htpB	1..296148	296148
	-	inside	IScluster/Tn	-	-	46415..66224	19809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T224448 WP_011251285.1 NZ_CP086762:c61415-61104 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224448 NZ_CP120329:3388414-3388632 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT224448 WP_011251286.1 NZ_CP086762:c61107-60688 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224448 NZ_CP120329:3388012-3388386 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1