Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 355141..355787 Replicon chromosome
Accession NZ_CP086761
Organism Klebsiella pneumoniae strain AZS099

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag LON84_RS01650 Protein ID WP_016529833.1
Coordinates 355141..355488 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LON84_RS01655 Protein ID WP_002920557.1
Coordinates 355488..355787 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LON84_RS01640 (351067) 351067..352500 + 1434 WP_023342584.1 glycogen synthase GlgA -
LON84_RS01645 (352518) 352518..354965 + 2448 WP_002920561.1 glycogen phosphorylase -
LON84_RS01650 (355141) 355141..355488 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LON84_RS01655 (355488) 355488..355787 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LON84_RS01660 (355850) 355850..357358 - 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
LON84_RS01665 (357563) 357563..357892 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LON84_RS01670 (357943) 357943..358773 + 831 WP_024622927.1 rhomboid family intramembrane serine protease GlpG -
LON84_RS01675 (358823) 358823..359581 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T224430 WP_016529833.1 NZ_CP086761:355141-355488 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T224430 NZ_CP120327:656066-656377 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT224430 WP_002920557.1 NZ_CP086761:355488-355787 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT224430 NZ_CP120327:656378-656668 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References