22441

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 279280..279821 Replicon chromosome
Accession NC_009801
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name yafQ Uniprot ID A7ZHY5
Locus tag ECE24377A_RS02905 Protein ID WP_012000916.1
Coordinates 279280..279558 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ECE24377A_RS02910 Protein ID WP_000729704.1
Coordinates 279561..279821 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS02890 276862..277440 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ECE24377A_RS02895 277646..278413 + 768 WP_000333380.1 class II glutamine amidotransferase -
ECE24377A_RS02900 278384..279124 - 741 WP_001225679.1 murein L,D-transpeptidase -
ECE24377A_RS02905 279280..279558 - 279 WP_012000916.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECE24377A_RS02910 279561..279821 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECE24377A_RS02915 280007..280780 + 774 WP_000543899.1 C40 family peptidase -
ECE24377A_RS02920 280837..281193 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
ECE24377A_RS02925 281186..281464 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
ECE24377A_RS02930 281569..283281 - 1713 Protein_245 flagellar biosynthesis protein FlhA -
ECE24377A_RS02935 283253..284038 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T22441 WP_012000916.1 NC_009801:c279558-279280 [Escherichia coli O139:H28 str. E24377A]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T22441 NC_009801:c279558-279280 [Escherichia coli O139:H28 str. E24377A]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT22441 WP_000729704.1 NC_009801:c279821-279561 [Escherichia coli O139:H28 str. E24377A]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT22441 NC_009801:c279821-279561 [Escherichia coli O139:H28 str. E24377A]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZHY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References