Detailed information of TA system TA224354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3222850..3223577
• Replicon: chromosome
• Accession: NZ_CP086678
• Organism: Escherichia coli strain EFF60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: LMF34_RS15405
• Protein ID: WP_001521139.1
• Coordinates: 3222850..3223161 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LMF34_RS15410
• Protein ID: WP_000126294.1
• Coordinates: 3223158..3223577 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMF34_RS15380 (3218785)	3218785..3220494	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
LMF34_RS15385 (3220504)	3220504..3221046	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LMF34_RS15390 (3221046)	3221046..3221813	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LMF34_RS15395 (3221810)	3221810..3222220	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LMF34_RS15400 (3222213)	3222213..3222683	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LMF34_RS15405 (3222850)	3222850..3223161	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
LMF34_RS15410 (3223158)	3223158..3223577	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LMF34_RS15415 (3223656)	3223656..3225080	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
LMF34_RS15420 (3225089)	3225089..3226546	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LMF34_RS15425 (3226806)	3226806..3227816	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
LMF34_RS15430 (3227965)	3227965..3228492	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T224354 WP_001521139.1 NZ_CP086678:3222850-3223161 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224354 NZ_CP120212:c2192443-2192141 [Agrobacterium leguminum]
ATGCAGCTAAAGCAATCGGCGACATTTCAAAAATGGTTTGGGAAGTTAAAGGATGAACGGGGCAAGGCTCTGATTACGTC
GCGACTGAACAGACTTTCCTTTGGTCATGCAGGCGATGTCGCTCCCGTTGGTGAGGGCGTCAGCGAATTGCGCATCCATT
ACGGACCGGGCTATCGGATCTATTTCGTCAGGTCGGGCGATGTCGTTATTGTTCTGCTCTGTGGCGGAGACAAGAGTACG
CAGGAGAAGGACATCAAAGCCGCGAAGCAGATTGCGGCGCAATGGAGCGATGACGATGATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT224354 WP_000126294.1 NZ_CP086678:3223158-3223577 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224354 NZ_CP120212:c2192148-2191858 [Agrobacterium leguminum]
ATGATTGAGAAGTTTACGAACTTTGATCCCGCCGAACACCTCAAGAGCGATGAAGCCATTGAGGTGTTTCTGGAGGATGC
GTTCGCAACCAATGATGCGGCATACATCGCAAATGCGCTTGGTGTTATCGCGCGCGCAAAGGGTATGACGCAGGTAGCGC
GTGAAACCGGACTTGCCAGAGAGCATCTTTACAAATCGCTCAGCGAGGCTGGGAATCCGACGTTAGAAACAACGATAGCC
GTCCTCAACTCCCTGGGCTTTCAGCTCACCAGCAAACACAAGGCGGCATGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9