Detailed information of TA system TA224351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2698322..2699121
• Replicon: chromosome
• Accession: NZ_CP086678
• Organism: Escherichia coli strain EFF60

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: LMF34_RS12920
• Protein ID: WP_000347272.1
• Coordinates: 2698322..2698786 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: LMF34_RS12925
• Protein ID: WP_001307405.1
• Coordinates: 2698786..2699121 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMF34_RS12890 (2693323)	2693323..2693757	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
LMF34_RS12895 (2693775)	2693775..2694653	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
LMF34_RS12900 (2694643)	2694643..2695422	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LMF34_RS12905 (2695433)	2695433..2695906	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LMF34_RS12910 (2695929)	2695929..2697209	-	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LMF34_RS12915 (2697458)	2697458..2698267	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
LMF34_RS12920 (2698322)	2698322..2698786	-	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LMF34_RS12925 (2698786)	2698786..2699121	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LMF34_RS12930 (2699270)	2699270..2700841	-	1572	WP_248422651.1	galactarate dehydratase	-
LMF34_RS12935 (2701216)	2701216..2702550	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
LMF34_RS12940 (2702566)	2702566..2703336	+	771	WP_213847718.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2698322..2709773	11451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T224351 WP_000347272.1 NZ_CP086678:c2698786-2698322 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T224351 NZ_CP120211:c1195459-1195169 [Agrobacterium leguminum]
GTGAATAGCTATCGGCTGACTAAACAGGCCGAAAGCGAAATTCTCGATATCTTTATCTATGGCGTTGAACAGTTCGGATT
GAGACAGGCACATTTTTACAAGAATGAAATGAAAAGCTGTTTCCAGTTGCTTGGCGATAATCCGCGCATGGGACGTCTTG
CCCCGGCGATTGGGGAAGGTGTACGCCGACATGAGCATGGAAGCCATATCATCCTCTATGAAATTGACGGTGCAGGCGTC
CTCATTCTCACGGTGGTGCATGGTCGAAGCATCCGCCGCCTGAAGCTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT224351 WP_001307405.1 NZ_CP086678:c2699121-2698786 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT224351 NZ_CP120211:c1195718-1195452 [Agrobacterium leguminum]
ATGGCGACCATGACCGTTTCCTTGCCAGATCCCATGAAAGAATGGATCGAGACCCAGGTCCTGACAGGCGAATATGCCAG
CGTTAGCGACTATGTGCGGGATCTGGTGCGTCGTGACAGGGCGCGGCGAGGGCAGGAGTTCAGCGTTGATGAGCTTCGCC
AGATCGTCGCGGATTCCCGCGCGAGCGGCATCGGTTCCCGCAGCATGGATGATCTGTTTACGGAAGCCGAACGGATAGCG
GCGGCACGTGGAGTTGTGCGTGAATAG

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7