Detailed information of TA system TA224350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1878452..1879054
• Replicon: chromosome
• Accession: NZ_CP086678
• Organism: Escherichia coli strain EFF60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LMF34_RS08985
• Protein ID: WP_000897305.1
• Coordinates: 1878743..1879054 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LMF34_RS08980
• Protein ID: WP_000356395.1
• Coordinates: 1878452..1878742 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMF34_RS08950 (1874075)	1874075..1874977	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LMF34_RS08955 (1874974)	1874974..1875609	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LMF34_RS08960 (1875606)	1875606..1876535	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LMF34_RS08965 (1876717)	1876717..1876959	-	243	WP_001306649.1	protein YiiF	-
LMF34_RS08970 (1877178)	1877178..1877396	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LMF34_RS08975 (1877815)	1877815..1878093	-	279	WP_001306650.1	hypothetical protein	-
LMF34_RS08980 (1878452)	1878452..1878742	-	291	WP_000356395.1	NadS family protein	Antitoxin
LMF34_RS08985 (1878743)	1878743..1879054	-	312	WP_000897305.1	hypothetical protein	Toxin
LMF34_RS08990 (1879283)	1879283..1880191	+	909	WP_001306651.1	alpha/beta hydrolase	-
LMF34_RS08995 (1880360)	1880360..1881274	-	915	WP_077634137.1	transposase	-
LMF34_RS09000 (1881287)	1881287..1882174	-	888	Protein_1755	hypothetical protein	-
LMF34_RS09005 (1882590)	1882590..1883531	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LMF34_RS09010 (1883576)	1883576..1884013	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T224350 WP_000897305.1 NZ_CP086678:c1879054-1878743 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224350 NZ_CP120211:790530-790928 [Agrobacterium leguminum]
ATGCTCACATATATGCTCGATACCAATATATGTATCTATGTGATGAAGACTTATCCTCCGGAGCTGCGGGAGAAATTCAA
CGCTCTGGCAGATCAACTGTGTATTTCGAGCATCACACTTGGCGAGCTCTATTATGGTGCTGAAAAATCCGTCCGGCGCC
AGGAAAACATCAACGCCATTGAAAATTTTACCGCGCGTTTGGACGTTCTGCCTTTCGCCGACAAAGGCGCCGCACATTAC
GGACAGATCAGGGCCGAACTTGCCAAGGCTGGAACGCCATGCGGCGTTCATGATATGCAGATTGGTGGTCATGCCCGTAG
CGAGGGGCTGATCGTCGTAACCAATAATATGCGCGAGTTTGTCCGGATGCCCGGTTTGCGAGTTGAAAACTGGGTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224350 WP_000356395.1 NZ_CP086678:c1878742-1878452 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224350 NZ_CP120211:790297-790530 [Agrobacterium leguminum]
ATGGCTACGTCAACGGTTTTTATAAGCAATCGCAGTCAAGCTGTAAGGCTACCGAAGGCCGTGGCTTTTCCTGATGACGT
GCATAAGGTCGATGTTCTGAAAATCGGTCTCGCGCGTGTTATAGTGCCGCAGGGAAAGCGCTGGGACGACCTTTTTCTGA
CCGGCCCTCGTGCAACGGATGATTTTCTTTCCGTTCGCGAAGAGCCTGCATTGGATGAGCGCGAGCCCTTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7