22435

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3277072..3277799 Replicon chromosome
Accession NC_009800
Organism Escherichia coli HS

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECHS_RS16115 Protein ID WP_000550189.1
Coordinates 3277485..3277799 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECHS_RS16110 Protein ID WP_000560255.1
Coordinates 3277072..3277488 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHS_RS16100 (EcHS_A3262) 3272332..3274683 + 2352 WP_000695494.1 alpha-glucosidase -
ECHS_RS16105 (EcHS_A3263) 3275009..3277027 + 2019 WP_000121450.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECHS_RS16110 (EcHS_A3264) 3277072..3277488 - 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECHS_RS16115 (EcHS_A3265) 3277485..3277799 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECHS_RS16125 (EcHS_A3266) 3278084..3279220 - 1137 WP_000018663.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECHS_RS16130 (EcHS_A3267) 3279305..3279808 + 504 WP_001295542.1 M48 family metallopeptidase -
ECHS_RS16135 (EcHS_A3268) 3279885..3280568 + 684 WP_001183041.1 vancomycin high temperature exclusion protein -
ECHS_RS16140 (EcHS_A3269) 3280647..3281633 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T22435 WP_000550189.1 NC_009800:c3277799-3277485 [Escherichia coli HS]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T22435 NC_009800:c3277799-3277485 [Escherichia coli HS]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT22435 WP_000560255.1 NC_009800:c3277488-3277072 [Escherichia coli HS]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT22435 NC_009800:c3277488-3277072 [Escherichia coli HS]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGCTCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References