Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 997729..998423 | Replicon | chromosome |
| Accession | NZ_CP086678 | ||
| Organism | Escherichia coli strain EFF60 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | A0A0D8WHS4 |
| Locus tag | LMF34_RS04875 | Protein ID | WP_001521903.1 |
| Coordinates | 997729..998127 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | LMF34_RS04880 | Protein ID | WP_000554758.1 |
| Coordinates | 998130..998423 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (993559) | 993559..993639 | - | 81 | NuclAT_11 | - | - |
| - (993559) | 993559..993639 | - | 81 | NuclAT_11 | - | - |
| - (993559) | 993559..993639 | - | 81 | NuclAT_11 | - | - |
| - (993559) | 993559..993639 | - | 81 | NuclAT_11 | - | - |
| LMF34_RS04845 (992899) | 992899..994143 | - | 1245 | WP_248422846.1 | esterase FrsA | - |
| LMF34_RS04850 (994235) | 994235..994693 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LMF34_RS04855 (994954) | 994954..996411 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| LMF34_RS04860 (996468) | 996468..996820 | - | 353 | Protein_950 | peptide chain release factor H | - |
| LMF34_RS04865 (996816) | 996816..997022 | - | 207 | Protein_951 | RtcB family protein | - |
| LMF34_RS04870 (997267) | 997267..997719 | - | 453 | WP_023144376.1 | GNAT family N-acetyltransferase | - |
| LMF34_RS04875 (997729) | 997729..998127 | - | 399 | WP_001521903.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LMF34_RS04880 (998130) | 998130..998423 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LMF34_RS04885 (998475) | 998475..999530 | - | 1056 | WP_001226177.1 | DNA polymerase IV | - |
| LMF34_RS04890 (999601) | 999601..1000386 | - | 786 | WP_000207556.1 | putative lateral flagellar export/assembly protein LafU | - |
| LMF34_RS04895 (1000358) | 1000358..1002070 | + | 1713 | Protein_957 | flagellar biosynthesis protein FlhA | - |
| LMF34_RS04900 (1002149) | 1002149..1002502 | + | 354 | WP_024166466.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| LMF34_RS04905 (1002559) | 1002559..1003308 | - | 750 | WP_016233951.1 | C40 family peptidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15471.88 Da Isoelectric Point: 8.0949
>T224344 WP_001521903.1 NZ_CP086678:c998127-997729 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T224344 NZ_CP119987:263888-264304 [Salmonella enterica]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT224344 WP_000554758.1 NZ_CP086678:c998423-998130 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT224344 NZ_CP119987:263661-263891 [Salmonella enterica]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0D8WHS4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |