Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 16217..16874 | Replicon | plasmid pNDM-1 |
| Accession | NZ_CP086666 | ||
| Organism | Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a) | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | KFX87_RS27940 | Protein ID | WP_000270043.1 |
| Coordinates | 16524..16874 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | KFX87_RS27935 | Protein ID | WP_000124640.1 |
| Coordinates | 16217..16519 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFX87_RS27895 (KFX87_027890) | 11256..11843 | + | 588 | Protein_17 | hypothetical protein | - |
| KFX87_RS27900 (KFX87_027895) | 11899..12654 | - | 756 | WP_000194037.1 | IS21-like element ISAs29 family helper ATPase IstB | - |
| KFX87_RS27905 (KFX87_027900) | 12669..14210 | - | 1542 | WP_001274811.1 | IS21-like element ISAs29 family transposase | - |
| KFX87_RS27910 (KFX87_027905) | 14432..14731 | + | 300 | Protein_20 | hypothetical protein | - |
| KFX87_RS27915 (KFX87_027910) | 14757..14948 | + | 192 | WP_001270409.1 | hypothetical protein | - |
| KFX87_RS27920 (KFX87_027915) | 14951..15478 | + | 528 | WP_001210659.1 | thermonuclease family protein | - |
| KFX87_RS27925 (KFX87_027920) | 15536..15808 | + | 273 | WP_001043046.1 | HU family DNA-binding protein | - |
| KFX87_RS27930 (KFX87_027925) | 15897..16190 | + | 294 | WP_001239998.1 | hypothetical protein | - |
| KFX87_RS27935 (KFX87_027930) | 16217..16519 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| KFX87_RS27940 (KFX87_027935) | 16524..16874 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KFX87_RS27945 (KFX87_027940) | 17037..17585 | + | 549 | WP_001061573.1 | hypothetical protein | - |
| KFX87_RS27950 (KFX87_027945) | 17926..18120 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| KFX87_RS27955 (KFX87_027950) | 18131..18502 | + | 372 | WP_000516918.1 | hypothetical protein | - |
| KFX87_RS27960 (KFX87_027955) | 18495..18965 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| KFX87_RS27965 (KFX87_027960) | 18980..19315 | - | 336 | WP_000683477.1 | hypothetical protein | - |
| KFX87_RS27970 (KFX87_027965) | 19412..19900 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| KFX87_RS27975 (KFX87_027970) | 19903..20400 | + | 498 | WP_000062185.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / armA / msr(E) / mph(E) / dfrA12 / aadA2 / blaNDM-1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa | htpB | 1..174841 | 174841 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T224339 WP_000270043.1 NZ_CP086666:c16874-16524 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T224339 NZ_CP119987:14789-15121 [Salmonella enterica]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT224339 WP_000124640.1 NZ_CP086666:c16519-16217 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT224339 NZ_CP119987:14531-14788 [Salmonella enterica]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|