Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4359489..4360168 Replicon chromosome
Accession NZ_CP086662
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name higB Uniprot ID U9XN95
Locus tag LOE88_RS20885 Protein ID WP_000057540.1
Coordinates 4359489..4359791 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LOE88_RS20890 Protein ID WP_000806442.1
Coordinates 4359827..4360168 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE88_RS20865 (4354752) 4354752..4355972 - 1221 WP_001251608.1 fosmidomycin MFS transporter -
LOE88_RS20870 (4356190) 4356190..4357842 + 1653 WP_000771768.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
LOE88_RS20875 (4357879) 4357879..4358358 - 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LOE88_RS20880 (4358562) 4358562..4359356 - 795 WP_000365180.1 TraB/GumN family protein -
LOE88_RS20885 (4359489) 4359489..4359791 + 303 WP_000057540.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOE88_RS20890 (4359827) 4359827..4360168 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LOE88_RS20895 (4360226) 4360226..4362730 - 2505 WP_023155691.1 copper-exporting P-type ATPase CopA -
LOE88_RS20900 (4362992) 4362992..4363924 + 933 WP_023155692.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11781.35 Da        Isoelectric Point: 10.2638

>T224316 WP_000057540.1 NZ_CP086662:4359489-4359791 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T224316 NZ_CP119984:3488105-3488632 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACAATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT224316 WP_000806442.1 NZ_CP086662:4359827-4360168 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT224316 NZ_CP119984:3487818-3488108 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XN95

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References