Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4083242..4083783 Replicon chromosome
Accession NZ_CP086662
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LOE88_RS19530 Protein ID WP_000615976.1
Coordinates 4083242..4083520 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LOE88_RS19535 Protein ID WP_000729704.1
Coordinates 4083523..4083783 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE88_RS19515 (4080824) 4080824..4081402 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
LOE88_RS19520 (4081608) 4081608..4082375 + 768 WP_000333380.1 class II glutamine amidotransferase -
LOE88_RS19525 (4082346) 4082346..4083086 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LOE88_RS19530 (4083242) 4083242..4083520 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LOE88_RS19535 (4083523) 4083523..4083783 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LOE88_RS19540 (4083969) 4083969..4084742 + 774 WP_000543899.1 C40 family peptidase -
LOE88_RS19545 (4084799) 4084799..4085155 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LOE88_RS19550 (4085148) 4085148..4085426 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LOE88_RS19555 (4085531) 4085531..4087624 - 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
LOE88_RS19560 (4087608) 4087608..4088747 - 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T224314 WP_000615976.1 NZ_CP086662:c4083520-4083242 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T224314 NZ_CP119984:c2142633-2142349 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT224314 WP_000729704.1 NZ_CP086662:c4083783-4083523 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT224314 NZ_CP119984:c2142871-2142623 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGAGGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References