Detailed information of TA system TA224310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3186937..3187539
• Replicon: chromosome
• Accession: NZ_CP086662
• Organism: Escherichia coli strain E2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LOE88_RS15290
• Protein ID: WP_000897305.1
• Coordinates: 3186937..3187248 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOE88_RS15295
• Protein ID: WP_000356397.1
• Coordinates: 3187249..3187539 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE88_RS15260 (3181967)	3181967..3182752	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
LOE88_RS15265 (3182851)	3182851..3183450	+	600	WP_001295269.1	glucose-1-phosphatase	-
LOE88_RS15270 (3183444)	3183444..3184316	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LOE88_RS15275 (3184313)	3184313..3184750	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LOE88_RS15280 (3184795)	3184795..3185736	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LOE88_RS15285 (3185800)	3185800..3186708	-	909	WP_001162704.1	alpha/beta hydrolase	-
LOE88_RS15290 (3186937)	3186937..3187248	+	312	WP_000897305.1	hypothetical protein	Toxin
LOE88_RS15295 (3187249)	3187249..3187539	+	291	WP_000356397.1	NadS family protein	Antitoxin
LOE88_RS15300 (3188125)	3188125..3188343	+	219	WP_001295676.1	CopG family transcriptional regulator	-
LOE88_RS15305 (3188562)	3188562..3188804	+	243	WP_001086388.1	protein YiiF	-
LOE88_RS15310 (3189134)	3189134..3190063	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LOE88_RS15315 (3190060)	3190060..3190695	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LOE88_RS15320 (3190692)	3190692..3191594	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T224310 WP_000897305.1 NZ_CP086662:3186937-3187248 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224310 NZ_CP119983:263895-264311 [Salmonella enterica]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT224310 WP_000356397.1 NZ_CP086662:3187249-3187539 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224310 NZ_CP119983:263668-263898 [Salmonella enterica]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7