Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2394698..2395497 Replicon chromosome
Accession NZ_CP086662
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag LOE88_RS11435 Protein ID WP_000347267.1
Coordinates 2395033..2395497 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LOE88_RS11430 Protein ID WP_001307405.1
Coordinates 2394698..2395033 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE88_RS11415 (2390484) 2390484..2391254 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
LOE88_RS11420 (2391270) 2391270..2392604 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LOE88_RS11425 (2392979) 2392979..2394550 + 1572 WP_001273741.1 galactarate dehydratase -
LOE88_RS11430 (2394698) 2394698..2395033 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LOE88_RS11435 (2395033) 2395033..2395497 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LOE88_RS11440 (2395552) 2395552..2396361 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LOE88_RS11445 (2396610) 2396610..2397890 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
LOE88_RS11450 (2397913) 2397913..2398386 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LOE88_RS11455 (2398397) 2398397..2399176 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LOE88_RS11460 (2399166) 2399166..2400044 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
LOE88_RS11465 (2400062) 2400062..2400496 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2384043..2395497 11454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T224308 WP_000347267.1 NZ_CP086662:2395033-2395497 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T224308 NZ_CP119983:50117-50422 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT224308 WP_001307405.1 NZ_CP086662:2394698-2395033 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT224308 NZ_CP119983:49897-50115 [Salmonella enterica]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References