Detailed information of TA system TA224277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4995944..4996546
• Replicon: chromosome
• Accession: NZ_CP086627
• Organism: Escherichia coli strain RIVM_C036569

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN356_RS24170
• Protein ID: WP_059332311.1
• Coordinates: 4996235..4996546 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN356_RS24165
• Protein ID: WP_000356395.1
• Coordinates: 4995944..4996234 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN356_RS24130 (4991568)	4991568..4992470	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN356_RS24135 (4992467)	4992467..4993102	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN356_RS24140 (4993099)	4993099..4994028	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN356_RS24145 (4994210)	4994210..4994452	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN356_RS24150 (4994671)	4994671..4994889	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN356_RS24155 (4995308)	4995308..4995586	-	279	WP_001315112.1	hypothetical protein	-
LN356_RS24160 (4995638)	4995638..4995859	-	222	WP_001550354.1	hypothetical protein	-
LN356_RS24165 (4995944)	4995944..4996234	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN356_RS24170 (4996235)	4996235..4996546	-	312	WP_059332311.1	hypothetical protein	Toxin
LN356_RS24175 (4996775)	4996775..4997683	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN356_RS24180 (4997851)	4997851..4998765	-	915	WP_109553727.1	transposase	-
LN356_RS24185 (4998778)	4998778..4999665	-	888	Protein_4732	hypothetical protein	-
LN356_RS24190 (5000081)	5000081..5001022	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN356_RS24195 (5001067)	5001067..5001504	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224277 WP_059332311.1 NZ_CP086627:c4996546-4996235 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224277 NZ_CP119980:443574-443858 [Salmonella enterica]
ATGAATTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224277 WP_000356395.1 NZ_CP086627:c4996234-4995944 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224277 NZ_CP119980:443342-443584 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCCTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGAATTA
TGA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6