Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4113161..4113855 Replicon chromosome
Accession NZ_CP086627
Organism Escherichia coli strain RIVM_C036569

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag LN356_RS19985 Protein ID WP_001263491.1
Coordinates 4113161..4113559 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag LN356_RS19990 Protein ID WP_000554755.1
Coordinates 4113562..4113855 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4108990) 4108990..4109070 - 81 NuclAT_10 - -
- (4108990) 4108990..4109070 - 81 NuclAT_10 - -
- (4108990) 4108990..4109070 - 81 NuclAT_10 - -
- (4108990) 4108990..4109070 - 81 NuclAT_10 - -
LN356_RS19955 (4108330) 4108330..4109574 - 1245 WP_000189541.1 esterase FrsA -
LN356_RS19960 (4109666) 4109666..4110124 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LN356_RS19965 (4110385) 4110385..4111842 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
LN356_RS19970 (4111899) 4111899..4112251 - 353 Protein_3918 peptide chain release factor H -
LN356_RS19975 (4112247) 4112247..4112453 - 207 Protein_3919 RtcB family protein -
LN356_RS19980 (4112699) 4112699..4113151 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
LN356_RS19985 (4113161) 4113161..4113559 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LN356_RS19990 (4113562) 4113562..4113855 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LN356_RS19995 (4113907) 4113907..4114962 - 1056 WP_001550655.1 DNA polymerase IV -
LN356_RS20000 (4115033) 4115033..4115818 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
LN356_RS20005 (4115790) 4115790..4117502 + 1713 Protein_3925 flagellar biosynthesis protein FlhA -
LN356_RS20010 (4117607) 4117607..4117885 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LN356_RS20015 (4117878) 4117878..4118234 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4103097..4113855 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T224273 WP_001263491.1 NZ_CP086627:c4113559-4113161 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T224273 NZ_CP119967:c1922738-1922163 [Pseudomonas sp. A34-9]
GTGAGGCATTCCTCTTTCACAGCTGTATACGATGCATGTGTTTTGTACCCCGCCCCTTTGAGAGACTTCCTGATGTGGCT
TGCCCTCTCGGGGCGGTTCCGGGCGCGATGGAGCAAGGAGATTCACAGCGAATGGAAACGCAATCTATTGAAGAACCGCA
CTGACCTGACCATGGAACAACTGGATCGTACGTCTGAATTGATGGACCGCGCGATCCCCGAAGCTTGCGTCTGCGACTAC
GAAGAACTGGTCAACGGACTGTCATTGCCCGATCAGGATGACCGTCATGTACTGGCGGCAGCCATTCGCTGTGGCGCAGG
CGTCATCGTGACGTTCAACTTGAAAGACTTTCCCGATACGCAGCTCGCCCCTTTTGGTATCGAAGCACAGCATCCCGATG
AGTTCGTCGAAAACCTGTTCCATCTTGATTCGGCAGCAGTTGTCTCTGCCGCCCAAAAGCAGCGAAAACAACTGAAAACG
CCAGCGATGGATGTCGAGTCGTTTTTGCGCTTACTGCAAAGACAAGGATTGACCCAGTCGGTCCAAGCGCTTGAAGGTTA
TCGCGCCATTCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT224273 WP_000554755.1 NZ_CP086627:c4113855-4113562 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT224273 NZ_CP119967:c1923202-1922735 [Pseudomonas sp. A34-9]
ATGTCGATCATTACCCATTCACCAATCAACCTACCTGTAGAGCGCGAAGTCAAAGCCGCCATCGAAGGCCAACGCGCCTT
GGCCGCTTACCTCGCGACTCAGTTTGAAACTCAGCACATCCAGATTTTCGATGAGCAAAACGAAGCTCACAGCATCGAAC
TGCCCACCTCGGCCTTGCGACTTCTCACCGACATTCTCGCGGCATTGGCTGAAGGCAATGCCGTCAAGGTCGTGCCGATT
CATGCCGAACTGACCACTCAGGAAGCCGCTGACCTGCTCAATGTCTCACGCCCGCACATGATTAAACTGCTGGAAAGTGG
CGAGATTTCCTACCACAAAACCGGCAAGCACCGACGTGTGCGCTTTGCCGATTTGATGGACTATAAGACCCGGCGAGAGA
CTGCCAGCGAACAGGCGATGGCGCTACTCGCAGAGCAGGCTCAGGCATTAAGGACAGGATACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References