Detailed information of TA system TA224250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5027357..5027959
• Replicon: chromosome
• Accession: NZ_CP086626
• Organism: Escherichia coli strain RIVM_C037134

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN362_RS24415
• Protein ID: WP_059332311.1
• Coordinates: 5027648..5027959 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN362_RS24410
• Protein ID: WP_000356395.1
• Coordinates: 5027357..5027647 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN362_RS24375 (5022981)	5022981..5023883	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN362_RS24380 (5023880)	5023880..5024515	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN362_RS24385 (5024512)	5024512..5025441	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN362_RS24390 (5025623)	5025623..5025865	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN362_RS24395 (5026084)	5026084..5026302	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN362_RS24400 (5026721)	5026721..5026999	-	279	WP_001315112.1	hypothetical protein	-
LN362_RS24405 (5027051)	5027051..5027272	-	222	WP_001550354.1	hypothetical protein	-
LN362_RS24410 (5027357)	5027357..5027647	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN362_RS24415 (5027648)	5027648..5027959	-	312	WP_059332311.1	hypothetical protein	Toxin
LN362_RS24420 (5028188)	5028188..5029096	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN362_RS24425 (5029264)	5029264..5030178	-	915	WP_109553727.1	transposase	-
LN362_RS24430 (5030191)	5030191..5031078	-	888	Protein_4781	hypothetical protein	-
LN362_RS24435 (5031494)	5031494..5032435	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN362_RS24440 (5032480)	5032480..5032917	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224250 WP_059332311.1 NZ_CP086626:c5027959-5027648 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224250 NZ_CP119862:807691-808104 [Citrobacter sp. S171]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCACAGTGGCTGTCCTTGCTTCTTCATGGGTTGGTCGCCGC
ATTGATTTTGTTGATGCCCTGGCCATTGAGCTATACCCCTCTGTGGCTGATATTGCTCTCACTGGTGGTGTTCGATTGCG
TACGCAGTCAGCGACGCATCAATACCCGTCAGGGCGAGATTAAATTGCTGATGGATGGGCGTTTGCGCTGGCAGGGGCTG
GAGTGGGCGATTCTGAGCGCACCCTGGATGCTCAAGACGGGCATGATGATGCGTTTGCGTTCGGATAGCGGTCGACGTCA
ACACTTATGGTTGGCGGCGGATAGTATGGACGAAAAAGAATGGCGTGAGTTACGTCGGGTAATGTTGCAGCAAACGGAAC
AAGAGAAGCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224250 WP_000356395.1 NZ_CP086626:c5027647-5027357 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224250 NZ_CP119862:807444-807710 [Citrobacter sp. S171]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCATGCCGTCGTGGTATGCGTGAGCTCGACATTTCCATCATGCCGTT
TTTCGAACATGAGTACGATAGTCTCAGTGATGATGAAAAACGCATTTTTATTCGTTTGCTGGAATGCGATGATCCGGATT
TATTCAACTGGTTAATGAATCATGGTAAGCCGGCAGATGCAGAACTGGAACAGATGGTGAGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6