Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4142832..4143526 | Replicon | chromosome |
| Accession | NZ_CP086626 | ||
| Organism | Escherichia coli strain RIVM_C037134 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | LN362_RS20235 | Protein ID | WP_001263491.1 |
| Coordinates | 4142832..4143230 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | LN362_RS20240 | Protein ID | WP_000554755.1 |
| Coordinates | 4143233..4143526 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4138661) | 4138661..4138741 | - | 81 | NuclAT_10 | - | - |
| - (4138661) | 4138661..4138741 | - | 81 | NuclAT_10 | - | - |
| - (4138661) | 4138661..4138741 | - | 81 | NuclAT_10 | - | - |
| - (4138661) | 4138661..4138741 | - | 81 | NuclAT_10 | - | - |
| LN362_RS20205 (4138001) | 4138001..4139245 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| LN362_RS20210 (4139337) | 4139337..4139795 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LN362_RS20215 (4140056) | 4140056..4141513 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| LN362_RS20220 (4141570) | 4141570..4141922 | - | 353 | Protein_3966 | peptide chain release factor H | - |
| LN362_RS20225 (4141918) | 4141918..4142124 | - | 207 | Protein_3967 | RtcB family protein | - |
| LN362_RS20230 (4142370) | 4142370..4142822 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| LN362_RS20235 (4142832) | 4142832..4143230 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LN362_RS20240 (4143233) | 4143233..4143526 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LN362_RS20245 (4143578) | 4143578..4144633 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| LN362_RS20250 (4144704) | 4144704..4145489 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| LN362_RS20255 (4145461) | 4145461..4147173 | + | 1713 | Protein_3973 | flagellar biosynthesis protein FlhA | - |
| LN362_RS20260 (4147278) | 4147278..4147556 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| LN362_RS20265 (4147549) | 4147549..4147905 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4132768..4143526 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T224246 WP_001263491.1 NZ_CP086626:c4143230-4142832 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T224246 NZ_CP119773:3363960-3364217 [Dickeya fangzhongdai]
ATGCCTGAGATTGACAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTACGATGATGAACGGCACCACTTGCCGCATGT
ACATGTCTGCTACGGCAACCATGAGCTGATCATCAGTATTCGTACTGGAACAGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGCTGGCGCTTGCGCATATCAGAGCCCATCGGGAACAACTGCTGAGTATGTGGGAGCGGGCGGTTGTGGGGAGA
AATCCGGGAAAACTGTGA
ATGCCTGAGATTGACAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTACGATGATGAACGGCACCACTTGCCGCATGT
ACATGTCTGCTACGGCAACCATGAGCTGATCATCAGTATTCGTACTGGAACAGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGCTGGCGCTTGCGCATATCAGAGCCCATCGGGAACAACTGCTGAGTATGTGGGAGCGGGCGGTTGTGGGGAGA
AATCCGGGAAAACTGTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT224246 WP_000554755.1 NZ_CP086626:c4143526-4143233 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT224246 NZ_CP119773:3364225-3364713 [Dickeya fangzhongdai]
ATGCATAAAATCGTGGAAGTTGACGTGATTGGCGACTTTTCGTTGCGCCTGACCTATGAGGATGGTTTGGTTTGCGACAT
CAATCTTTATCAATATGCCTCCAGCCCGGTATTTTCGAACGCCAGCCTATTTATCAAGTTTGGGCTGCTGCCGGATGGTT
CGCTGGAATGGTCGCCGGATATTAAGATTTCCGCGTCGGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTTGCG
GTGCGAGAAAAGAACTTCGCTGATGTCATCTCTCGCGCCTTCTATGATGCCGTGATTGAGAACCGGCCGGAAATTCTGCA
AGCGGCGTTGAAAATCGCGGTGGAAAAACATGGCAACAGCACAGTAGCCAAAGAGGCGGGGGCGAAAAGTCGCACCAGTC
TGTATAAGTCGCTGAATAAAAATACCCATGTCAGTCTTGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAACTGGAA
GAGAAGTAA
ATGCATAAAATCGTGGAAGTTGACGTGATTGGCGACTTTTCGTTGCGCCTGACCTATGAGGATGGTTTGGTTTGCGACAT
CAATCTTTATCAATATGCCTCCAGCCCGGTATTTTCGAACGCCAGCCTATTTATCAAGTTTGGGCTGCTGCCGGATGGTT
CGCTGGAATGGTCGCCGGATATTAAGATTTCCGCGTCGGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTTGCG
GTGCGAGAAAAGAACTTCGCTGATGTCATCTCTCGCGCCTTCTATGATGCCGTGATTGAGAACCGGCCGGAAATTCTGCA
AGCGGCGTTGAAAATCGCGGTGGAAAAACATGGCAACAGCACAGTAGCCAAAGAGGCGGGGGCGAAAAGTCGCACCAGTC
TGTATAAGTCGCTGAATAAAAATACCCATGTCAGTCTTGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAACTGGAA
GAGAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |