Detailed information of TA system TA224231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1303323..1304050
• Replicon: chromosome
• Accession: NZ_CP086626
• Organism: Escherichia coli strain RIVM_C037134

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: LN362_RS06320
• Protein ID: WP_000547555.1
• Coordinates: 1303323..1303634 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN362_RS06325
• Protein ID: WP_000126294.1
• Coordinates: 1303631..1304050 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN362_RS06295 (1299258)	1299258..1300967	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
LN362_RS06300 (1300977)	1300977..1301519	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LN362_RS06305 (1301519)	1301519..1302286	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LN362_RS06310 (1302283)	1302283..1302693	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LN362_RS06315 (1302686)	1302686..1303156	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
LN362_RS06320 (1303323)	1303323..1303634	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
LN362_RS06325 (1303631)	1303631..1304050	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LN362_RS06330 (1304129)	1304129..1305553	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
LN362_RS06335 (1305562)	1305562..1307019	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LN362_RS06340 (1307279)	1307279..1308289	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
LN362_RS06345 (1308438)	1308438..1308965	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T224231 WP_000547555.1 NZ_CP086626:1303323-1303634 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224231 NZ_CP119766:1186181-1186477 [Flavobacterium sp. CJ75]
ATGAATTATAAGATTAGTATAGAAGCATCTTATGATTTAGAAAAAATTTGGCTTTATACTTTTGATACTTGGTCTGCCGA
ACAAGCAGACAGGTATTTAAAATTGATATTGGATGAAATAGAATACTTGTGTTTAAAACCTAATTCAGGAAAAGATTTCA
GCCATATCAGAAAAGGTTATTTTAGAACTAAAGTAAAGTCACATTTAATATTTTATAAAATAAACATCAAACAAAACGAA
ATAGAGATTATTAGAGTTTTGCACCAAATGATGGATATTGAAAACCACCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT224231 WP_000126294.1 NZ_CP086626:1303631-1304050 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224231 NZ_CP119766:1185937-1186191 [Flavobacterium sp. CJ75]
ATGGCAAAGAATACATCAATATTGTTAGGAGATTATTTTGATAATTTTATTAATTCACAAGTAAAAACAGGTCGATTTAC
ATCTGCAAGTGAGGTAATTCGGGCTGCATTGAGAATGTTTGAAGAAGAACAAACCAAAAGTGAAGAACTAATAAAAGAAT
TAAAAAAAGGAGAAAAATCAGGATTTGTAAAAGATTTTGATCGTGAATCTTTTATAAAAAACCTTCACGAAAAACATATT
AAAAATGAATTATAA

Structures

Toxin

• AlphaFold DB: V0YLE2