Detailed information of TA system TA224223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5051146..5051748
• Replicon: chromosome
• Accession: NZ_CP086625
• Organism: Escherichia coli strain RIVM_C037166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN360_RS24595
• Protein ID: WP_059332311.1
• Coordinates: 5051437..5051748 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN360_RS24590
• Protein ID: WP_000356395.1
• Coordinates: 5051146..5051436 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN360_RS24555 (5046770)	5046770..5047672	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN360_RS24560 (5047669)	5047669..5048304	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN360_RS24565 (5048301)	5048301..5049230	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN360_RS24570 (5049412)	5049412..5049654	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN360_RS24575 (5049873)	5049873..5050091	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN360_RS24580 (5050510)	5050510..5050788	-	279	WP_001315112.1	hypothetical protein	-
LN360_RS24585 (5050840)	5050840..5051061	-	222	WP_001550354.1	hypothetical protein	-
LN360_RS24590 (5051146)	5051146..5051436	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN360_RS24595 (5051437)	5051437..5051748	-	312	WP_059332311.1	hypothetical protein	Toxin
LN360_RS24600 (5051977)	5051977..5052885	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN360_RS24605 (5053053)	5053053..5053967	-	915	WP_109553727.1	transposase	-
LN360_RS24610 (5053980)	5053980..5054867	-	888	Protein_4814	hypothetical protein	-
LN360_RS24615 (5055283)	5055283..5056224	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN360_RS24620 (5056269)	5056269..5056706	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224223 WP_059332311.1 NZ_CP086625:c5051748-5051437 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224223 NZ_CP119754:c2309344-2309021 [Superficieibacter sp. HKU1]
ATGCATGTAATTTCACAAAAGAAACTTGCCCTTGCGGCAGAGAAGTACCCACAGTTCAGAGATGAGTTGATCGCAATGGG
AAAGATTATTGCTAAAGGGTATTTCCCTGGCCCTGATTCATTACGGCAAATTTATCCCACTCTGGATAATATTAAATATA
TTGATAAGTACTATGTTATTGATATAGCAAGAAACAGGCTACGAGTCATTGCAATTATCTTCTTCAGGACACAGAAATTT
TATGTTCGACATGTTTACACACATAAAGAGTACGATGAATTTGTGATGAAGTGCCGCACTAAAGGAAAAACCAAATGGCT
GTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224223 WP_000356395.1 NZ_CP086625:c5051436-5051146 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224223 NZ_CP119754:c2309030-2308614 [Superficieibacter sp. HKU1]
ATGGCTGTAGAAGAAGCATTAAAAGCAGCAGAAGCAATAAAACGAGCAATCCCCCTCCTGGTCGGTAGTACACGATGTGA
GGACTATGATAATGCTCTGAGTCTGGTTGAATATTGGCTCCTGCACGACCCGGAAAACCCGCTGCTTGAGTTGGTAGCCG
CAAAAATTGCGGCATATGAAGCAACATTACCTGAAGTGGCTGCATTCAGAAAAGCGAGCGCTGAAGCCCCTTCCGCACTC
GCTACGCTACGGATCCTCATCGACCAACATAATCTGACGCTTTCAGATTTTAAAGATGAAATCGGCAGCAAATCTATGGT
ATCGCGCGTATTAAACGGTGAACGCCAACTTACCCTCGCACACATAAAAAAACTTGCTGCACGGTTTGGTGTATCCCCAA
CGCTATTCCTCGACTAA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6