Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4171278..4171972 | Replicon | chromosome |
| Accession | NZ_CP086625 | ||
| Organism | Escherichia coli strain RIVM_C037166 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | LN360_RS20450 | Protein ID | WP_001263491.1 |
| Coordinates | 4171278..4171676 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | LN360_RS20455 | Protein ID | WP_000554755.1 |
| Coordinates | 4171679..4171972 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4167107) | 4167107..4167187 | - | 81 | NuclAT_10 | - | - |
| - (4167107) | 4167107..4167187 | - | 81 | NuclAT_10 | - | - |
| - (4167107) | 4167107..4167187 | - | 81 | NuclAT_10 | - | - |
| - (4167107) | 4167107..4167187 | - | 81 | NuclAT_10 | - | - |
| LN360_RS20420 (4166447) | 4166447..4167691 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| LN360_RS20425 (4167783) | 4167783..4168241 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LN360_RS20430 (4168502) | 4168502..4169959 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| LN360_RS20435 (4170016) | 4170016..4170368 | - | 353 | Protein_4006 | peptide chain release factor H | - |
| LN360_RS20440 (4170364) | 4170364..4170570 | - | 207 | Protein_4007 | RtcB family protein | - |
| LN360_RS20445 (4170816) | 4170816..4171268 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| LN360_RS20450 (4171278) | 4171278..4171676 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LN360_RS20455 (4171679) | 4171679..4171972 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LN360_RS20460 (4172024) | 4172024..4173079 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| LN360_RS20465 (4173150) | 4173150..4173935 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| LN360_RS20470 (4173907) | 4173907..4175619 | + | 1713 | Protein_4013 | flagellar biosynthesis protein FlhA | - |
| LN360_RS20475 (4175724) | 4175724..4176002 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| LN360_RS20480 (4175995) | 4175995..4176351 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4161214..4171972 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T224219 WP_001263491.1 NZ_CP086625:c4171676-4171278 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T224219 NZ_CP119754:353394-353924 [Superficieibacter sp. HKU1]
GTGGACGAGTTAATTATTGAGCTTTTTGCTGACGCGACAGAATATAACCTCAACGAATTTGACTGCGGCGAAGAGACATT
AAACCGCTTTTTATTTGAACACCTCAGCCGTCAGCACCATAGAAAAATCCTGCGTGGCTATGTGCTCAGAACCACAGGGC
CTGTTCCTGCTGTTTTAGGGTACTACACGCTTTCCGGAAGCTGCTTTGAAAAAGCGTCTCTTCCCTCAAAAACACAGCAG
AAACAAATACCTTATCGAAACGTACCCAGCATCACGCTCGGGCGTCTGGCCGTAGACAAAAGGCTTCAGGGTCAGGGATG
GGGAACCATGCTTGTAACCCATGCTATGAAGGTTATTTTTGGCGCATCTCAGACTGTTGGCATCCACGGCATGTTTGTGG
AGGCGCTAAATGATAACGCCAGAGCATTTTATCTCAGCCTGGGGTTTATTCCGCTATCAGGCGAGAACAATCATGCGCTC
TTTTATCCTGTGAAATCCATTGAATCGCTTTTTGCGTCAGATATAGCGTAA
GTGGACGAGTTAATTATTGAGCTTTTTGCTGACGCGACAGAATATAACCTCAACGAATTTGACTGCGGCGAAGAGACATT
AAACCGCTTTTTATTTGAACACCTCAGCCGTCAGCACCATAGAAAAATCCTGCGTGGCTATGTGCTCAGAACCACAGGGC
CTGTTCCTGCTGTTTTAGGGTACTACACGCTTTCCGGAAGCTGCTTTGAAAAAGCGTCTCTTCCCTCAAAAACACAGCAG
AAACAAATACCTTATCGAAACGTACCCAGCATCACGCTCGGGCGTCTGGCCGTAGACAAAAGGCTTCAGGGTCAGGGATG
GGGAACCATGCTTGTAACCCATGCTATGAAGGTTATTTTTGGCGCATCTCAGACTGTTGGCATCCACGGCATGTTTGTGG
AGGCGCTAAATGATAACGCCAGAGCATTTTATCTCAGCCTGGGGTTTATTCCGCTATCAGGCGAGAACAATCATGCGCTC
TTTTATCCTGTGAAATCCATTGAATCGCTTTTTGCGTCAGATATAGCGTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT224219 WP_000554755.1 NZ_CP086625:c4171972-4171679 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT224219 NZ_CP119754:353121-353390 [Superficieibacter sp. HKU1]
ATGCCTGTACTAAAGAAACAGCGCATTGATCTCAGGCTCAGCGATGACGATAAAGGCATGATTGAAGAGGCGGCTGTACT
CACCAACCAGACAATCACTCAATTTATGCTGCGCAGCGCGTCTGAACGTGCCGCACAGGTGATTGAACAACATCGTCGGA
TGGTCCTGAATGATGAATCGTGGGCGCGTGTGATGGATGCACTCAGCAACCCACCTGAACCCAATGAAAAACTGAAAAAA
GCAGCCAGACGTCTACAAAACATGGAGTAA
ATGCCTGTACTAAAGAAACAGCGCATTGATCTCAGGCTCAGCGATGACGATAAAGGCATGATTGAAGAGGCGGCTGTACT
CACCAACCAGACAATCACTCAATTTATGCTGCGCAGCGCGTCTGAACGTGCCGCACAGGTGATTGAACAACATCGTCGGA
TGGTCCTGAATGATGAATCGTGGGCGCGTGTGATGGATGCACTCAGCAACCCACCTGAACCCAATGAAAAACTGAAAAAA
GCAGCCAGACGTCTACAAAACATGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |