Detailed information of TA system TA224204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1304907..1305634
• Replicon: chromosome
• Accession: NZ_CP086625
• Organism: Escherichia coli strain RIVM_C037166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: LN360_RS06345
• Protein ID: WP_000547555.1
• Coordinates: 1304907..1305218 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN360_RS06350
• Protein ID: WP_000126294.1
• Coordinates: 1305215..1305634 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN360_RS06320 (1300842)	1300842..1302551	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
LN360_RS06325 (1302561)	1302561..1303103	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LN360_RS06330 (1303103)	1303103..1303870	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LN360_RS06335 (1303867)	1303867..1304277	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LN360_RS06340 (1304270)	1304270..1304740	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
LN360_RS06345 (1304907)	1304907..1305218	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
LN360_RS06350 (1305215)	1305215..1305634	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LN360_RS06355 (1305713)	1305713..1307137	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
LN360_RS06360 (1307146)	1307146..1308603	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LN360_RS06365 (1308863)	1308863..1309873	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
LN360_RS06370 (1310022)	1310022..1310549	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T224204 WP_000547555.1 NZ_CP086625:1304907-1305218 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224204 NZ_CP119746:3885321-3885599 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT224204 WP_000126294.1 NZ_CP086625:1305215-1305634 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224204 NZ_CP119746:3885058-3885318 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: V0YLE2