Detailed information of TA system TA224196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5039114..5039716
• Replicon: chromosome
• Accession: NZ_CP086624
• Organism: Escherichia coli strain RIVM_C037791

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN354_RS24505
• Protein ID: WP_059332311.1
• Coordinates: 5039405..5039716 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN354_RS24500
• Protein ID: WP_000356395.1
• Coordinates: 5039114..5039404 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN354_RS24465 (5034738)	5034738..5035640	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN354_RS24470 (5035637)	5035637..5036272	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN354_RS24475 (5036269)	5036269..5037198	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN354_RS24480 (5037380)	5037380..5037622	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN354_RS24485 (5037841)	5037841..5038059	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN354_RS24490 (5038478)	5038478..5038756	-	279	WP_001315112.1	hypothetical protein	-
LN354_RS24495 (5038808)	5038808..5039029	-	222	WP_001550354.1	hypothetical protein	-
LN354_RS24500 (5039114)	5039114..5039404	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN354_RS24505 (5039405)	5039405..5039716	-	312	WP_059332311.1	hypothetical protein	Toxin
LN354_RS24510 (5039945)	5039945..5040853	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN354_RS24515 (5041021)	5041021..5041935	-	915	WP_109553727.1	transposase	-
LN354_RS24520 (5041948)	5041948..5042835	-	888	Protein_4798	hypothetical protein	-
LN354_RS24525 (5043251)	5043251..5044192	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN354_RS24530 (5044237)	5044237..5044674	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224196 WP_059332311.1 NZ_CP086624:c5039716-5039405 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224196 NZ_CP119746:2189675-2189778 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224196 WP_000356395.1 NZ_CP086624:c5039404-5039114 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224196 NZ_CP119746:c2189816-2189545 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6