Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4153016..4153710 | Replicon | chromosome |
| Accession | NZ_CP086624 | ||
| Organism | Escherichia coli strain RIVM_C037791 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | LN354_RS20300 | Protein ID | WP_001263491.1 |
| Coordinates | 4153016..4153414 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | LN354_RS20305 | Protein ID | WP_000554755.1 |
| Coordinates | 4153417..4153710 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4148845) | 4148845..4148925 | - | 81 | NuclAT_10 | - | - |
| - (4148845) | 4148845..4148925 | - | 81 | NuclAT_10 | - | - |
| - (4148845) | 4148845..4148925 | - | 81 | NuclAT_10 | - | - |
| - (4148845) | 4148845..4148925 | - | 81 | NuclAT_10 | - | - |
| LN354_RS20270 (4148185) | 4148185..4149429 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| LN354_RS20275 (4149521) | 4149521..4149979 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LN354_RS20280 (4150240) | 4150240..4151697 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| LN354_RS20285 (4151754) | 4151754..4152106 | - | 353 | Protein_3978 | peptide chain release factor H | - |
| LN354_RS20290 (4152102) | 4152102..4152308 | - | 207 | Protein_3979 | RtcB family protein | - |
| LN354_RS20295 (4152554) | 4152554..4153006 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| LN354_RS20300 (4153016) | 4153016..4153414 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LN354_RS20305 (4153417) | 4153417..4153710 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LN354_RS20310 (4153762) | 4153762..4154817 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| LN354_RS20315 (4154888) | 4154888..4155673 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| LN354_RS20320 (4155645) | 4155645..4157357 | + | 1713 | Protein_3985 | flagellar biosynthesis protein FlhA | - |
| LN354_RS20325 (4157462) | 4157462..4157740 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| LN354_RS20330 (4157733) | 4157733..4158089 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4142952..4153710 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T224192 WP_001263491.1 NZ_CP086624:c4153414-4153016 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T224192 NZ_CP119746:1071991-1072326 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT224192 WP_000554755.1 NZ_CP086624:c4153710-4153417 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT224192 NZ_CP119746:1071743-1071991 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |