Detailed information of TA system TA224177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1304161..1304888
• Replicon: chromosome
• Accession: NZ_CP086624
• Organism: Escherichia coli strain RIVM_C037791

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: LN354_RS06340
• Protein ID: WP_000547555.1
• Coordinates: 1304161..1304472 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN354_RS06345
• Protein ID: WP_000126294.1
• Coordinates: 1304469..1304888 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN354_RS06315 (1300096)	1300096..1301805	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
LN354_RS06320 (1301815)	1301815..1302357	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LN354_RS06325 (1302357)	1302357..1303124	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LN354_RS06330 (1303121)	1303121..1303531	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LN354_RS06335 (1303524)	1303524..1303994	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
LN354_RS06340 (1304161)	1304161..1304472	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
LN354_RS06345 (1304469)	1304469..1304888	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LN354_RS06350 (1304967)	1304967..1306391	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
LN354_RS06355 (1306400)	1306400..1307857	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LN354_RS06360 (1308117)	1308117..1309127	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
LN354_RS06365 (1309276)	1309276..1309803	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T224177 WP_000547555.1 NZ_CP086624:1304161-1304472 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224177 NZ_CP119740:4615924-4616301 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT224177 WP_000126294.1 NZ_CP086624:1304469-1304888 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224177 NZ_CP119740:4615503-4615877 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: V0YLE2