Detailed information of TA system TA224169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5044921..5045523
• Replicon: chromosome
• Accession: NZ_CP086623
• Organism: Escherichia coli strain RIVM_C037794

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN358_RS24530
• Protein ID: WP_059332311.1
• Coordinates: 5045212..5045523 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN358_RS24525
• Protein ID: WP_000356395.1
• Coordinates: 5044921..5045211 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN358_RS24490 (5040545)	5040545..5041447	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN358_RS24495 (5041444)	5041444..5042079	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN358_RS24500 (5042076)	5042076..5043005	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN358_RS24505 (5043187)	5043187..5043429	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN358_RS24510 (5043648)	5043648..5043866	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN358_RS24515 (5044285)	5044285..5044563	-	279	WP_001315112.1	hypothetical protein	-
LN358_RS24520 (5044615)	5044615..5044836	-	222	WP_001550354.1	hypothetical protein	-
LN358_RS24525 (5044921)	5044921..5045211	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN358_RS24530 (5045212)	5045212..5045523	-	312	WP_059332311.1	hypothetical protein	Toxin
LN358_RS24535 (5045752)	5045752..5046660	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN358_RS24540 (5046828)	5046828..5047742	-	915	WP_109553727.1	transposase	-
LN358_RS24545 (5047755)	5047755..5048642	-	888	Protein_4802	hypothetical protein	-
LN358_RS24550 (5049058)	5049058..5049999	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN358_RS24555 (5050044)	5050044..5050481	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224169 WP_059332311.1 NZ_CP086623:c5045523-5045212 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224169 NZ_CP119740:c1976916-1976542 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224169 WP_000356395.1 NZ_CP086623:c5045211-5044921 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224169 NZ_CP119740:c1977310-1977005 [Escherichia coli]
CTGCCCTGTACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGC
CGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAC
TCATGCTCACCAGCGGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCC
GATACCCTCAGCAGTTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6