22416

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 275519..276060 Replicon chromosome
Accession NC_009800
Organism Escherichia coli HS

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag ECHS_RS01260 Protein ID WP_000615976.1
Coordinates 275519..275797 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ECHS_RS01265 Protein ID WP_000729704.1
Coordinates 275800..276060 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHS_RS01245 (EcHS_A0250) 273101..273679 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ECHS_RS01250 (EcHS_A0251) 273885..274652 + 768 WP_000333380.1 class II glutamine amidotransferase -
ECHS_RS01255 (EcHS_A0252) 274623..275363 - 741 WP_001225679.1 murein L,D-transpeptidase -
ECHS_RS01260 (EcHS_A0253) 275519..275797 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECHS_RS01265 (EcHS_A0254) 275800..276060 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECHS_RS01270 (EcHS_A0255) 276246..277019 + 774 WP_000543897.1 C40 family peptidase -
ECHS_RS01275 (EcHS_A0256) 277076..277432 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
ECHS_RS01280 (EcHS_A0257) 277425..277703 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
ECHS_RS01285 (EcHS_A0259) 277808..279520 - 1713 Protein_241 flagellar biosynthesis protein FlhA -
ECHS_RS01290 (EcHS_A0258) 279492..280277 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T22416 WP_000615976.1 NC_009800:c275797-275519 [Escherichia coli HS]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T22416 NC_009800:c275797-275519 [Escherichia coli HS]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT22416 WP_000729704.1 NC_009800:c276060-275800 [Escherichia coli HS]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT22416 NC_009800:c276060-275800 [Escherichia coli HS]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References