Detailed information of TA system TA224142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5059116..5059718
• Replicon: chromosome
• Accession: NZ_CP086622
• Organism: Escherichia coli strain RIVM_C037884

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN352_RS24615
• Protein ID: WP_059332311.1
• Coordinates: 5059407..5059718 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN352_RS24610
• Protein ID: WP_000356395.1
• Coordinates: 5059116..5059406 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN352_RS24575 (5054740)	5054740..5055642	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN352_RS24580 (5055639)	5055639..5056274	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN352_RS24585 (5056271)	5056271..5057200	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN352_RS24590 (5057382)	5057382..5057624	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN352_RS24595 (5057843)	5057843..5058061	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN352_RS24600 (5058480)	5058480..5058758	-	279	WP_001315112.1	hypothetical protein	-
LN352_RS24605 (5058810)	5058810..5059031	-	222	WP_001550354.1	hypothetical protein	-
LN352_RS24610 (5059116)	5059116..5059406	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN352_RS24615 (5059407)	5059407..5059718	-	312	WP_059332311.1	hypothetical protein	Toxin
LN352_RS24620 (5059947)	5059947..5060855	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN352_RS24625 (5061023)	5061023..5061937	-	915	WP_109553727.1	transposase	-
LN352_RS24630 (5061950)	5061950..5062837	-	888	Protein_4819	hypothetical protein	-
LN352_RS24635 (5063253)	5063253..5064194	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN352_RS24640 (5064239)	5064239..5064676	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224142 WP_059332311.1 NZ_CP086622:c5059718-5059407 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224142 NZ_CP119734:c2913311-2913000 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224142 WP_000356395.1 NZ_CP086622:c5059406-5059116 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224142 NZ_CP119734:c2913003-2912584 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6