Detailed information of TA system TA022413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2850613..2851340
• Replicon: chromosome
• Accession: NC_009792
• Organism: Citrobacter koseri ATCC BAA-895

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A8AL02
• Locus tag: CKO_RS13130
• Protein ID: WP_012133872.1
• Coordinates: 2851032..2851340 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CKO_RS13125
• Protein ID: WP_012133871.1
• Coordinates: 2850613..2851032 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKO_RS13100	2845809..2846738	+	930	WP_012133862.1	1,4-dihydroxy-2-naphthoate polyprenyltransferase	-
CKO_RS13105	2846831..2847316	+	486	WP_012133864.1	ribonuclease E activity regulator RraA	-
CKO_RS13110	2847482..2847727	-	246	WP_012133867.1	septal ring assembly protein ZapB	-
CKO_RS13115	2848118..2848963	+	846	WP_012133869.1	aquaporin	-
CKO_RS13120	2848987..2850495	+	1509	WP_012133870.1	glycerol kinase GlpK	-
CKO_RS13125	2850613..2851032	-	420	WP_012133871.1	helix-turn-helix domain-containing protein	Antitoxin
CKO_RS13130	2851032..2851340	-	309	WP_012133872.1	type II toxin-antitoxin system HigB family toxin	Toxin
CKO_RS13135	2851481..2852491	+	1011	WP_012133873.1	class II fructose-bisphosphatase	-
CKO_RS13140	2852587..2853333	+	747	WP_012133874.1	ferredoxin--NADP(+) reductase	-
CKO_RS13145	2853409..2853855	-	447	WP_012133875.1	DUF805 domain-containing protein	-
CKO_RS13150	2853956..2854552	+	597	WP_012133876.1	YiiQ family protein	-
CKO_RS13155	2854667..2855434	+	768	WP_024130662.1	triose-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 12368.15 Da
• Isoelectric Point: 10.0486

>T22413 WP_012133872.1 NC_009792:c2851340-2851032 [Citrobacter koseri ATCC BAA-895]
MHVISKEPFEEAAKRYPNDSLAIRSLYRLMRETDFSSPTEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHISTHAEYDKLTRYYRENK

Nucleotide

• Length: 309 bp

>T22413 NC_009792:c2851340-2851032 [Citrobacter koseri ATCC BAA-895]
ATGCACGTCATTTCAAAAGAACCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATACGGTCGCTGTA
CCGCTTAATGCGTGAAACGGACTTCTCTTCTCCGACTGAGATGAGAACTCTGATACCGAGCCTGGACAATTTTAAGTACC
GGAACAAATGGTGGGTTCTGGATGTGGGAGGCAACAACTTGAGGGTTATCGCCTACATCAATTTTGTGAATAAACGCTTC
TATGTGAAGCACATCAGCACCCACGCTGAATATGACAAGTTGACCCGCTATTACAGGGAGAACAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15380.38 Da
• Isoelectric Point: 4.3485

>AT22413 WP_012133871.1 NC_009792:c2851032-2850613 [Citrobacter koseri ATCC BAA-895]
MNTDTAKAIEATKQLVAAVPFLGGSSSESDYREALSLVDYLIENDDENPLIDFLASKIADYEDNSDRFAEFNKGIAEMPV
GVALLRTLIDQYKLSYSDLKEEIGSKSLVSQILSGQRSLTIAHIKALSTRFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT22413 NC_009792:c2851032-2850613 [Citrobacter koseri ATCC BAA-895]
ATGAATACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTCGCCGCTGTTCCTTTCCTGGGAGGCAGCTCTTC
AGAGAGCGATTACCGTGAAGCGCTGTCTCTGGTGGATTATCTGATCGAGAACGACGATGAGAACCCGCTCATTGACTTCC
TGGCGAGCAAAATTGCCGACTATGAGGATAACAGCGACCGTTTCGCTGAGTTCAATAAAGGTATCGCTGAAATGCCGGTT
GGCGTGGCGCTGCTACGTACTCTGATTGACCAGTACAAGCTGTCCTACTCAGACCTGAAAGAGGAGATCGGTTCAAAGTC
GCTGGTGAGTCAGATCTTATCTGGTCAGCGCTCTCTGACTATCGCTCACATTAAGGCACTCTCTACTCGCTTCGGTGTGA
AGCCAGAGTGGTTTCTTTAA

Structures

Toxin

• AlphaFold DB: A8AL02