Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1303259..1303986 Replicon chromosome
Accession NZ_CP086622
Organism Escherichia coli strain RIVM_C037884

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag LN352_RS06315 Protein ID WP_000547555.1
Coordinates 1303259..1303570 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LN352_RS06320 Protein ID WP_000126294.1
Coordinates 1303567..1303986 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN352_RS06290 (1299194) 1299194..1300903 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
LN352_RS06295 (1300913) 1300913..1301455 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
LN352_RS06300 (1301455) 1301455..1302222 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
LN352_RS06305 (1302219) 1302219..1302629 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
LN352_RS06310 (1302622) 1302622..1303092 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
LN352_RS06315 (1303259) 1303259..1303570 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
LN352_RS06320 (1303567) 1303567..1303986 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
LN352_RS06325 (1304065) 1304065..1305489 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
LN352_RS06330 (1305498) 1305498..1306955 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
LN352_RS06335 (1307215) 1307215..1308225 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
LN352_RS06340 (1308374) 1308374..1308901 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T224123 WP_000547555.1 NZ_CP086622:1303259-1303570 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T224123 NZ_CP119732:116407-116556 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT224123 WP_000126294.1 NZ_CP086622:1303567-1303986 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT224123 NZ_CP119732:c116363-116302 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References