Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 675023..675822 Replicon chromosome
Accession NZ_CP086622
Organism Escherichia coli strain RIVM_C037884

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag LN352_RS03265 Protein ID WP_000347270.1
Coordinates 675023..675487 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag LN352_RS03270 Protein ID WP_001551693.1
Coordinates 675487..675822 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN352_RS03235 (670024) 670024..670458 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LN352_RS03240 (670476) 670476..671354 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LN352_RS03245 (671344) 671344..672123 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LN352_RS03250 (672134) 672134..672607 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LN352_RS03255 (672630) 672630..673910 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
LN352_RS03260 (674159) 674159..674968 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LN352_RS03265 (675023) 675023..675487 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LN352_RS03270 (675487) 675487..675822 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LN352_RS03275 (675971) 675971..677542 - 1572 WP_001273738.1 galactarate dehydratase -
LN352_RS03280 (677917) 677917..679251 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LN352_RS03285 (679267) 679267..680037 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T224118 WP_000347270.1 NZ_CP086622:c675487-675023 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T224118 NZ_CP119730:3896568-3896846 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT224118 WP_001551693.1 NZ_CP086622:c675822-675487 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT224118 NZ_CP119730:3896305-3896565 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References