Detailed information of TA system TA224110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5027959..5028561
• Replicon: chromosome
• Accession: NZ_CP086620
• Organism: Escherichia coli strain RIVM_C039205

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN351_RS24430
• Protein ID: WP_059332311.1
• Coordinates: 5028250..5028561 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN351_RS24425
• Protein ID: WP_000356395.1
• Coordinates: 5027959..5028249 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN351_RS24390 (5023583)	5023583..5024485	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN351_RS24395 (5024482)	5024482..5025117	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN351_RS24400 (5025114)	5025114..5026043	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN351_RS24405 (5026225)	5026225..5026467	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN351_RS24410 (5026686)	5026686..5026904	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN351_RS24415 (5027323)	5027323..5027601	-	279	WP_001315112.1	hypothetical protein	-
LN351_RS24420 (5027653)	5027653..5027874	-	222	WP_001550354.1	hypothetical protein	-
LN351_RS24425 (5027959)	5027959..5028249	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN351_RS24430 (5028250)	5028250..5028561	-	312	WP_059332311.1	hypothetical protein	Toxin
LN351_RS24435 (5028790)	5028790..5029698	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN351_RS24440 (5029866)	5029866..5030780	-	915	WP_109553727.1	transposase	-
LN351_RS24445 (5030793)	5030793..5031680	-	888	Protein_4782	hypothetical protein	-
LN351_RS24450 (5032096)	5032096..5033037	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN351_RS24455 (5033082)	5033082..5033519	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224110 WP_059332311.1 NZ_CP086620:c5028561-5028250 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224110 NZ_CP119730:2189676-2189779 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224110 WP_000356395.1 NZ_CP086620:c5028249-5027959 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224110 NZ_CP119730:c2189817-2189546 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6