Detailed information of TA system TA224082

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90874..91143
• Replicon: plasmid pRIVM_C039227_1
• Accession: NZ_CP086619
• Organism: Escherichia coli strain RIVM_C039227

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LN355_RS25820
• Protein ID: WP_001372321.1
• Coordinates: 91018..91143 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90874..90939 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN355_RS25780	85949..86197	+	249	WP_071606928.1	hypothetical protein	-
LN355_RS25785	86667..87194	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
LN355_RS25790	87250..87483	+	234	WP_000006018.1	DUF905 domain-containing protein	-
LN355_RS25795	87542..89500	+	1959	WP_032152921.1	ParB/RepB/Spo0J family partition protein	-
LN355_RS25800	89555..89989	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
LN355_RS25805	89986..90748	+	763	Protein_118	plasmid SOS inhibition protein A	-
LN355_RS25810	90717..90905	-	189	WP_001299721.1	hypothetical protein	-
-	90717..90941	+	225	NuclAT_0	-	-
-	90717..90941	+	225	NuclAT_0	-	-
-	90717..90941	+	225	NuclAT_0	-	-
-	90717..90941	+	225	NuclAT_0	-	-
-	90874..90939	-	66	-	-	Antitoxin
LN355_RS25815	90927..91076	+	150	Protein_120	plasmid maintenance protein Mok	-
LN355_RS25820	91018..91143	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LN355_RS25825	91363..91593	+	231	WP_071587244.1	hypothetical protein	-
LN355_RS25830	91591..91764	-	174	Protein_123	hypothetical protein	-
LN355_RS25835	92062..92349	+	288	WP_000107537.1	hypothetical protein	-
LN355_RS25840	92470..93291	+	822	WP_001234469.1	DUF932 domain-containing protein	-
LN355_RS25845	93588..94235	-	648	WP_124760006.1	transglycosylase SLT domain-containing protein	-
LN355_RS25850	94521..94904	+	384	WP_001151564.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LN355_RS25855	95098..95784	+	687	WP_000332487.1	PAS domain-containing protein	-
LN355_RS25860	95878..96105	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A)	-	1..126075	126075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T224082 WP_001372321.1 NZ_CP086619:91018-91143 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T224082 NZ_CP119726:1923263-1923514 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT224082 NZ_CP086619:c90939-90874 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin