Detailed information of TA system TA224079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5006955..5007557
• Replicon: chromosome
• Accession: NZ_CP086618
• Organism: Escherichia coli strain RIVM_C039227

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN355_RS24255
• Protein ID: WP_059332311.1
• Coordinates: 5007246..5007557 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN355_RS24250
• Protein ID: WP_000356395.1
• Coordinates: 5006955..5007245 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN355_RS24215 (5002579)	5002579..5003481	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN355_RS24220 (5003478)	5003478..5004113	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN355_RS24225 (5004110)	5004110..5005039	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN355_RS24230 (5005221)	5005221..5005463	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN355_RS24235 (5005682)	5005682..5005900	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN355_RS24240 (5006319)	5006319..5006597	-	279	WP_001315112.1	hypothetical protein	-
LN355_RS24245 (5006649)	5006649..5006870	-	222	WP_001550354.1	hypothetical protein	-
LN355_RS24250 (5006955)	5006955..5007245	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN355_RS24255 (5007246)	5007246..5007557	-	312	WP_059332311.1	hypothetical protein	Toxin
LN355_RS24260 (5007786)	5007786..5008694	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN355_RS24265 (5008862)	5008862..5009776	-	915	WP_109553727.1	transposase	-
LN355_RS24270 (5009789)	5009789..5010676	-	888	Protein_4746	hypothetical protein	-
LN355_RS24275 (5011092)	5011092..5012033	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN355_RS24280 (5012078)	5012078..5012515	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224079 WP_059332311.1 NZ_CP086618:c5007557-5007246 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224079 NZ_CP119726:926242-926649 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224079 WP_000356395.1 NZ_CP086618:c5007245-5006955 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224079 NZ_CP119726:925995-926261 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6