Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4122395..4123089 | Replicon | chromosome |
| Accession | NZ_CP086618 | ||
| Organism | Escherichia coli strain RIVM_C039227 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | LN355_RS20070 | Protein ID | WP_001263491.1 |
| Coordinates | 4122395..4122793 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | LN355_RS20075 | Protein ID | WP_000554755.1 |
| Coordinates | 4122796..4123089 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4118224) | 4118224..4118304 | - | 81 | NuclAT_10 | - | - |
| - (4118224) | 4118224..4118304 | - | 81 | NuclAT_10 | - | - |
| - (4118224) | 4118224..4118304 | - | 81 | NuclAT_10 | - | - |
| - (4118224) | 4118224..4118304 | - | 81 | NuclAT_10 | - | - |
| LN355_RS20040 (4117564) | 4117564..4118808 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| LN355_RS20045 (4118900) | 4118900..4119358 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LN355_RS20050 (4119619) | 4119619..4121076 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| LN355_RS20055 (4121133) | 4121133..4121485 | - | 353 | Protein_3932 | peptide chain release factor H | - |
| LN355_RS20060 (4121481) | 4121481..4121687 | - | 207 | Protein_3933 | RtcB family protein | - |
| LN355_RS20065 (4121933) | 4121933..4122385 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| LN355_RS20070 (4122395) | 4122395..4122793 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LN355_RS20075 (4122796) | 4122796..4123089 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LN355_RS20080 (4123141) | 4123141..4124196 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| LN355_RS20085 (4124267) | 4124267..4125052 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| LN355_RS20090 (4125024) | 4125024..4126736 | + | 1713 | Protein_3939 | flagellar biosynthesis protein FlhA | - |
| LN355_RS20095 (4126841) | 4126841..4127119 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| LN355_RS20100 (4127112) | 4127112..4127468 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4112331..4123089 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T224075 WP_001263491.1 NZ_CP086618:c4122793-4122395 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T224075 NZ_CP119678:1605704-1606039 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT224075 WP_000554755.1 NZ_CP086618:c4123089-4122796 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT224075 NZ_CP119678:1605427-1605714 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |