Detailed information of TA system TA224060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1303300..1304027
• Replicon: chromosome
• Accession: NZ_CP086618
• Organism: Escherichia coli strain RIVM_C039227

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: LN355_RS06320
• Protein ID: WP_000547555.1
• Coordinates: 1303300..1303611 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN355_RS06325
• Protein ID: WP_000126294.1
• Coordinates: 1303608..1304027 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN355_RS06295 (1299235)	1299235..1300944	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
LN355_RS06300 (1300954)	1300954..1301496	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LN355_RS06305 (1301496)	1301496..1302263	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LN355_RS06310 (1302260)	1302260..1302670	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LN355_RS06315 (1302663)	1302663..1303133	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
LN355_RS06320 (1303300)	1303300..1303611	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
LN355_RS06325 (1303608)	1303608..1304027	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LN355_RS06330 (1304106)	1304106..1305530	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
LN355_RS06335 (1305539)	1305539..1306996	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LN355_RS06340 (1307256)	1307256..1308266	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
LN355_RS06345 (1308415)	1308415..1308942	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T224060 WP_000547555.1 NZ_CP086618:1303300-1303611 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224060 NZ_CP119581:c41437-41057 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGATATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTAGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCCCCTGAACTGGCAGACCTTACTGTAGGGGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT224060 WP_000126294.1 NZ_CP086618:1303608-1304027 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224060 NZ_CP119581:c41658-41437 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0YLE2